anti-Human STAT1 antibody for Immunocytochemistry

Recommended STAT1 Antibody (supplied by: Log in to see )

Antigen
Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) Antibodies
  • CANDF7
  • ISGF-3
  • STAT91
  • 2010005J02Rik
  • AA408197
  • XStat1
  • STAT4
  • DD6G4-4
  • STAT
  • STAT1
  • dZ182H3.3
  • si:by134g18.2
  • si:by51f19.1
  • si:dz182h3.3
  • si:dz199m19.1
  • si:dz73o4.2
  • stat1
  • signal transducer and activator of transcription 1
  • signal transducer and activator of transcription 1 L homeolog
  • signal transducer and activator of transcription 1, 91kDa
  • signal transducer and activator of transcription 4
  • signal transducer and activator of transcription 1a
  • STAT1
  • Stat1
  • stat1.L
  • stat4
  • stat1a
Epitope
pSer727
107
79
55
21
18
16
15
10
9
8
8
7
6
6
6
5
5
5
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
569
311
255
42
32
27
26
23
18
15
11
10
9
5
4
3
3
2
2
2
2
1
1
Host
Rabbit
432
152
9
3
1
1
Clonality
Polyclonal
Conjugate
This STAT1 antibody is un-conjugated
16
15
12
11
10
10
7
5
5
5
4
4
4
4
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
481
225
160
115
103
53
52
28
27
12
9
7
5
5
2
1
1
1
Supplier
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Catalog No. ABIN6256410
$ 390.77
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
17.061531 ABIN4898979 CyTOF FACS ICC Rat IgG2b AA 1-194 Log in to see 246523 0
14.5 ABIN6255991 ELISA ICC IF IHC IP WB Rabbit IgG pTyr701 Log in to see Polyclonal 2
14.5 ABIN6265311 ELISA ICC IF IHC IP WB Rabbit IgG Log in to see Polyclonal 2
12.755764 ABIN5620877 ICC IF IHC IP WB Rabbit C-Term Log in to see Polyclonal 0
12.755764 ABIN931271 DB ICC Mouse IgG1 Log in to see 177C2a 0
10 ABIN214518 ICC IHC IHC (p) IP WB Rabbit IgG C-Term Log in to see Polyclonal 0
10 ABIN6265313 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0
10 ABIN1860646 ICC IHC WB Rabbit IgG AA 343-602 Log in to see Polyclonal 0
7 ABIN1030701 ICC IP ELISA WB Rabbit C-Term Log in to see Polyclonal 0
7 ABIN1739689 ICC IHC (p) IP WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN1832284 DB ICC Mouse IgG1 Log in to see 177C2a 0
1 ABIN2628793 ICC IF IHC IHC (p) IP WB Rabbit pSer727 Log in to see Polyclonal 0
1 ABIN2886255 ICC IHC WB Rabbit pSer727 Log in to see Polyclonal 0
1 ABIN2572800 DB ICC WB Mouse IgG1 Log in to see 177C2a 0
1 ABIN2766820 ICC IHC (p) IP WB Rabbit C-Term Log in to see Polyclonal 0
1 ABIN1887413 ICC IP ELISA WB Rabbit C-Term Log in to see Polyclonal 0
1 ABIN2959935 ICC IP ELISA WB Rabbit IgG Alpha Chain, C-Term Log in to see Polyclonal 0
1 ABIN1870633 ICC IHC WB Rabbit IgG pSer727 Log in to see Polyclonal 0

General

Antigen Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) Antibodies
Epitope pSer727
(107), (79), (55), (21), (18), (16), (15), (10), (9), (8), (8), (7), (6), (6), (6), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(569), (311), (255), (42), (32), (27), (26), (23), (18), (15), (11), (10), (9), (5), (4), (3), (3), (2), (2), (2), (2), (1), (1)
Host Rabbit
(432), (152), (9), (3), (1), (1)
Clonality
Polyclonal
Conjugate This STAT1 antibody is un-conjugated
(16), (15), (12), (11), (10), (10), (7), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(481), (225), (160), (115), (103), (53), (52), (28), (27), (12), (9), (7), (5), (5), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-STAT1 Antibody

Target Details STAT1 Application Details Handling References for anti-STAT1 antibody (ABIN6256410) Images
Specificity Phospho-STAT1 (Ser727) Antibody detects endogenous levels of STAT1 only when phosphorylated at Serine 727
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Purification The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Immunogen A synthesized peptide derived from human STAT1 around the phosphorylation site of Serine 727
Isotype IgG
Plasmids, Primers & others

Target Details STAT1

Product Details anti-STAT1 Antibody Application Details Handling References for anti-STAT1 antibody (ABIN6256410) Images back to top
Antigen
Alternative Name STAT1 (STAT1 Antibody Abstract)
Background

Description: Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus (PubMed:28753426). ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated (PubMed:26479788). It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4.

Gene: STAT1

Molecular Weight 91kDa
Gene ID 6772
UniProt P42224
Pathways JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, CXCR4-mediated Signaling Events

Application Details

Product Details anti-STAT1 Antibody Target Details STAT1 Handling References for anti-STAT1 antibody (ABIN6256410) Images back to top
Application Notes WB 1:500-1:2000 IHC 1:50-1:200, IF/ICC 1:100-1:500
Restrictions For Research Use only

Handling

Product Details anti-STAT1 Antibody Target Details STAT1 Application Details References for anti-STAT1 antibody (ABIN6256410) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months

References for anti-STAT1 antibody (ABIN6256410)

Product Details anti-STAT1 Antibody Target Details STAT1 Application Details Handling Images back to top
Product cited in:

Wu, Chen, Jing, Gu, Mei, Yao, Zhou, Yang, Sun, Wang, Hu, Wüthrich, Mei: "The C-terminal tail of polycystin-1 regulates complement factor B expression by signal transducer and activator of transcription 1." in: American journal of physiology. Renal physiology, Vol. 310, Issue 11, pp. F1284-94, 2017

Images

Product Details anti-STAT1 Antibody Target Details STAT1 Application Details Handling References for anti-STAT1 antibody (ABIN6256410) back to top
Supplier Images
Western Blotting (WB) image for anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (pSer727) antibody (ABIN6256410) Western blot analysis of STAT1 phosphorylation expression in Jurkat whole cell lysate...
Immunohistochemistry (IHC) image for anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (pSer727) antibody (ABIN6256410) ABIN6267508 at 1/200 staining Human lung cancer tissue sections by IHC-P. The tissue ...
Immunohistochemistry (IHC) image for anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (pSer727) antibody (ABIN6256410) ABIN6267508 at 1/200 staining Human bladder cancer tissue sections by IHC-P. The tiss...
Immunohistochemistry (IHC) image for anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (pSer727) antibody (ABIN6256410) ABIN6267508 at 1/200 staining Mouse testis tissue sections by IHC-P. The tissue was f...
Immunohistochemistry (IHC) image for anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (pSer727) antibody (ABIN6256410) ABIN6267508 at 1/200 staining Mouse lung tissue sections by IHC-P. The tissue was for...
Immunohistochemistry (IHC) image for anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (pSer727) antibody (ABIN6256410) ABIN6267508 at 1/200 staining Rat lung tissue sections by IHC-P. The tissue was forma...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (pSer727) antibody (ABIN6256410) ABIN6267508 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (pSer727) antibody (ABIN6256410) ABIN6267508 staining MCF7 by IF/ICC. The sample were fixed with PFA and permeabilized...