Coenzyme A Assay Kit

Catalog No. ABIN1000302

Product Details

Target: IVD (Isovaleryl-CoA Dehydrogenase (IVD))

Application: Biochemical Assay (BCA)

Specificity: 3 μM fatty acid

Characteristics: Sensitive. Use 10 µL samples. Linear detection range: colorimetric assay 5 - 1000 µM, fluorimetric assay 3 - 100 µM CoA.
Convenient. Room temperature mix-and-read procedure can be readily automated for high-throughput assay of thousands of samples per day.

Components: Assay Buffer: 20 mL. Dye Reagent: 120 µL. Enzyme A: 120 µL. Enzyme B: 120 µL. Substrate: 600 µL. Standard: 50 µL ATP: 120 µL.

Material not included: Pipetting devices, centrifuge tubes, clear flat-bottom uncoated 96-well plates, optical density plate reader, black flat-bottom uncoated 96-well plates, fluorescence plate reader.

Application Details

Application Notes: Assays: CoA in a variety of biological samples.

Calculation of Results:

Subtract Blank value (Standard #4) from the standard values and plot the OD or F against standard concentrations. Note: if the calculated CoA concentration of a sample is higher than 1000 µM in the Colorimetric Assay or 100 µM in the Fluorimetric Assay, dilute sample in Assay Buffer and repeat the assay. Multiply result by the dilution factor, n.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Target Details

Target: IVD (Isovaleryl-CoA Dehydrogenase (IVD))

Alternative Name: Coenzyme A (IVD Products)

Synonyms: 1300016K07Rik Kit, 6720455E18Rik Kit, AI463340 Kit, ACAD2 Kit, ATIVD Kit, ISOVALERYL-COA-DEHYDROGENASE Kit, ISOVALERYL-COA-DEHYDROGENASE PRECURSOR Kit, IVDH Kit, isovaleryl-CoA-dehydrogenase Kit, isovaleryl coenzyme A dehydrogenase Kit, isovaleryl-CoA dehydrogenase Kit, isovaleryl-CoA-dehydrogenase Kit, Ivd Kit, IVD Kit

Background: Quantitative determination of coenzyme A (CoA) by colorimetric (570nm) or fluorimetric (530/590nm) method.
Procedure: 60 min.

Coenzyme A (CoA) is involved in many important biological activities including the synthesis and oxidation of fatty acids, pyruvate oxidation in the citric acid cycle and many others. One of CoA's most crucial roles is the carrying and transferring of acyl groups. This method provides a simple, two-step and high-throughput assay for measuring CoA. In this assay, the first step enzymatically converts CoA to acyl-CoA and the second step oxidizes the acyl-CoA producing an enoyl-CoA and H2O2. The resulting H2O2 reacts with a specific dye to form a pink colored product. The optical density at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the CoA concentration in the sample.