Acetyl Coenzyme A (Acetyl-CoA) Fluorometric Assay Kit

Catalog No. ABIN7850029

Quick Overview for Acetyl Coenzyme A (Acetyl-CoA) Fluorometric Assay Kit (ABIN7850029)

Target: IVD (Isovaleryl-CoA Dehydrogenase (IVD))

Detection Method: Fluorometric

Application: Biochemical Assay (BCA)

Sample Type: Cell Samples, Plasma, Serum, Tissue Samples

Analytical Method: Quantitative

Product Details

Detection Range: 0.27 μmol/L - 5.00 μmol/L

Minimum Detection Limit: 0.27 μmol/L

Purpose: Acetyl Coenzyme A (Acetyl-CoA) Fluorometric Assay Kit

Characteristics: Acetyl-CoA is an essential cofactor and acyl carrier in the enzymatic acetyl transfer reaction. It can be formed by oxidative decarboxylation of pyruvate in mitochondria, oxidation of long-chain fatty acids, or oxidative degradation of certain amino acids. Acetyl-CoA is the starting compound of the citric acid cycle.
The detection principle of this kit is that Acetyl-CoA is converted into other substances under the condition of enzyme catalysis, and the resulting fluorescence is detected by fluorescence microplate reader at the maximum excitation wavelength of 535 nm and the maximum emission wavelength of 587 nm.

Application Details

Assay Time: 0.5 h

Assay Precision:

Inter-assay precision: 2.7-3.6%

Intra-assay precision: 1.7-4.1%

Restrictions: For Research Use only

Handling

Handling Advice: Protect from light.

Storage: -20 °C

Storage Comment: This product can be stored at -20°C for 12 months with shading light.

Expiry Date: 12 months

Target Details

Target: IVD (Isovaleryl-CoA Dehydrogenase (IVD))

Alternative Name: Coenzyme A