Phospholipase D Assay Kit

Catalog No. ABIN1000328

Quick Overview for Phospholipase D Assay Kit (ABIN1000328)

Target: Phospholipase D (PLD)

Application: Activity Assay (AcA)

Product Details

Specificity: 0.04 U/L

Characteristics: Sensitive. Use 10 µL samples. Detection range: colorimetric assay 0.06 - 10 U/L, fluorimetric assay 0.04 - 1 U/L.
Simple and High-throughput: the assay involves addition of a single working reagent and can be readily adapted to high-throughput assays for drug screening.

Components: Assay Buffer: 10 mL. Dye Reagent: 120 µL. Enzyme Mix: 120 µL. Substrate: 1.5 mL. Calibrator: 400 µL.

Material not included: Pipetting devices, centrifuge tubes, clear flat-bottom uncoated 96-well plates, optical density plate reader, black flat-bottom uncoated 96-well plates, fluorescence plate reader.

Application Details

Application Notes: Direct Assays: phospholipase D in biological samples.
Drug Discovery/Pharmacology: effects of drugs on phospholipase D metabolism.

Calculation of Results:

Subtract blank value (#4) from the standard values and plot the OD or F against standard concentrations.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Target Details

Target: Phospholipase D (PLD)

Alternative Name: Phospholipase D

Background: Quantitative determination of phospholipase D activity by colorimetric (570nm) or fluorimetric (530/590nm) method.
Procedure: 30 min.

Phospholipase D (PLD) catalyses the hydrolysis of the phosphodiester bond of glycerophospholipids to generate phosphatidic acid and a free headgroup. Abnormalities in PLD expression have been associated with human cancers. This method provides a simple and high-throughput assay for measuring PLD activity. In this assay, PLD hydrolyzes phosphatidylcholine to choline which is determined using choline oxidase and a H2O2 specific dye. The optical density of the pink colored product at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the PLD activity in the sample.