Phospholipase D (PLD) Activity Colorimetric Assay Kit

Catalog No. ABIN7850075

Quick Overview for Phospholipase D (PLD) Activity Colorimetric Assay Kit (ABIN7850075)

Target: Phospholipase D (PLD)

Detection Method: Colorimetric

Application: Biochemical Assay (BCA)

Sample Type: Animal, Plant Tissue, Plasma, Serum

Analytical Method: Quantitative

Product Details

Detection Range: 0.68 U/L - 16.73 U/L

Minimum Detection Limit: 0.68 U/L

Purpose: Phospholipase D (PLD) Activity Colorimetric Assay Kit

Characteristics: Phospholipase D (PLD) exists widely in natural plants and animals, and PLD plays a key role in phospholipase metabolism. PLD hydrolyzes phosphodiester bonds of glycerol phospholipids to produce phosphatidylic acid and nitrogenous base. Abnormal PLD activity and expression are associated with Alzheimer's disease, stroke, cancer, and other brain diseases.
The detection principle of this kit is that PLD catalyze the substrate and the chromogenic agent to product colored substance, which has the maximum absorption at the wavelength of 550 nm. By measuring the OD value at 550 nm, PLD activity in the sample is calculated according to the standard curve.

Application Details

Assay Time: 1 h

Assay Precision:

Inter-assay precision: 1.8-9.5%

Intra-assay precision: 1.7-2.8%

Restrictions: For Research Use only

Handling

Handling Advice: Protect from light.

Storage: -20 °C

Storage Comment: This product can be stored at -20°C for 12 months with shading light.

Expiry Date: 12 months

Target Details

Target: Phospholipase D (PLD)

Alternative Name: Phospholipase D