PPT1 ELISA Kit

Catalog No. ABIN1146897

The Human PPT1 ELISA Kit (ABIN1146897) is a Colorimetric ELISA Kit designed to quantify Human PPT1.

Quick Overview for PPT1 ELISA Kit (ABIN1146897)

Target: PPT1 (Palmitoyl-Protein Thioesterase 1 (PPT1))

Reactivity: Human

Detection Method: Colorimetric

Detection Range: 0.62-40.0 ng/mL

Application: ELISA

Product Details PPT1 ELISA Kit

Minimum Detection Limit: 0.62 ng/mL

Characteristics: The ELISA is based on the competitive binding enzyme immunoassaytechnique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PPT-1. During the reaction, PPT-1 in the sample or standard competes with a fixed amount of biotin-labeled PPT-1 for sites on a pre-coated Monoclonal antibody specific to PPT-1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PPT-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Components: Reagent (Quantity): Assay plate (1), Standard (2), Sample Diluent (1x20ml), Assay Diluent A (1x10ml), Assay Diluent B (1x10ml), Detection Reagent A (1x120µl), Detection Reagent B (1x120µl), Wash Buffer(25 x concentrate) (1x30ml), Substrate (1x10ml), Stop Solution (1x10ml)

Application Details

Comment:

Gene Name: PPT1

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Storage Comment: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Target Details for PPT1

Target: PPT1 (Palmitoyl-Protein Thioesterase 1 (PPT1))

Alternative Name: Palmitoyl-protein thioesterase 1

Background: Synonyms: Homo sapiens,Human,Palmitoyl-protein hydrolase 1,Palmitoyl-protein thioesterase 1,PPT,PPT1,PPT-1

Pathways: SARS-CoV-2 Protein Interactome