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EGFR ELISA Kit

EGFR Reactivity: Rat Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum
Catalog No. ABIN1569547
  • Target See all EGFR ELISA Kits
    EGFR (Epidermal Growth Factor Receptor (EGFR))
    Reactivity
    • 35
    • 25
    • 23
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    Rat
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.312 ng/mL - 20 ng/mL
    Minimum Detection Limit
    0.312 ng/mL
    Application
    ELISA
    Purpose
    The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of EGFR in rat serum, plasma and other biological fluids.
    Sample Type
    Plasma, Serum
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of this index.
    Cross-Reactivity (Details)
    No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
    Sensitivity
    0.125 ng/mL
    Components
    • Pre-coated, ready to use 96-well strip plate
    • Standard (freeze dried)
    • Standard Diluent
    • Detection Reagent A
    • Detection Reagent B
    • Assay Diluent A
    • Assay Diluent B
    • TMB
    • Stop Solution
    • Wash Buffer (30X)
    • Plate sealer for 96 wells
    • Instruction manual
    Material not included
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Sample Volume
    100 μL
    Assay Time
    1 - 4.5 h
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Assay Procedure

    The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

    Assay Precision
    • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
    • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
    • CV(%) = SD/meanX100
    • Intra-assay: CV<10%
    • Inter-assay: CV<12%
    Restrictions
    For Research Use only
  • Precaution of Use
    The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
    Handling Advice
    The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
    Storage
    4 °C,-20 °C
    Storage Comment
    The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
    Expiry Date
    12 months
  • Target See all EGFR ELISA Kits
    EGFR (Epidermal Growth Factor Receptor (EGFR))
    Alternative Name
    EGFR (EGFR Products)
    Synonyms
    C-erb ELISA Kit, CG10079 ELISA Kit, D-EGFR ELISA Kit, D-Egf ELISA Kit, DEGFR ELISA Kit, DER ELISA Kit, DER flb ELISA Kit, DER/EGFR ELISA Kit, DER/faint little ball ELISA Kit, DER/top ELISA Kit, DER/torpedo ELISA Kit, DER1 ELISA Kit, DEgfr ELISA Kit, Degfr ELISA Kit, Der ELISA Kit, DmHD-33 ELISA Kit, Dmel\\CG10079 ELISA Kit, EFG-R ELISA Kit, EGF-R ELISA Kit, EGFR ELISA Kit, EGFr ELISA Kit, EGfr ELISA Kit, EK2-6 ELISA Kit, Egf ELISA Kit, Egf-r ELISA Kit, EgfR ELISA Kit, El ELISA Kit, Elp ELISA Kit, Elp-1 ELISA Kit, Elp-B1 ELISA Kit, Elp-B1RB1 ELISA Kit, HD-33 ELISA Kit, TOP ELISA Kit, Torpedo/DER ELISA Kit, Torpedo/Egfr ELISA Kit, c-erbB ELISA Kit, d-egf-r ELISA Kit, dEGFR ELISA Kit, dEGFR1 ELISA Kit, dEgfr ELISA Kit, der ELISA Kit, egfr ELISA Kit, flb ELISA Kit, l(2)05351 ELISA Kit, l(2)09261 ELISA Kit, l(2)57DEFa ELISA Kit, l(2)57EFa ELISA Kit, l(2)57Ea ELISA Kit, mor1 ELISA Kit, top ELISA Kit, top/DER ELISA Kit, top/flb ELISA Kit, torpedo/Egfr ELISA Kit, torpedo/egfr ELISA Kit, EGFR12 ELISA Kit, EGFR15 ELISA Kit, egfr1 ELISA Kit, Erbb2 ELISA Kit, ERBB ELISA Kit, ERBB1 ELISA Kit, HER1 ELISA Kit, PIG61 ELISA Kit, mENA ELISA Kit, ErbB-1 ELISA Kit, Errp ELISA Kit, 9030024J15Rik ELISA Kit, AI552599 ELISA Kit, Erbb ELISA Kit, Errb1 ELISA Kit, Wa5 ELISA Kit, wa-2 ELISA Kit, wa2 ELISA Kit, epidermal growth factor receptor ELISA Kit, Epidermal growth factor receptor ELISA Kit, epidermal growth factor receptor a (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) ELISA Kit, EGFR ELISA Kit, Egfr ELISA Kit, egfra ELISA Kit, egfr1 ELISA Kit, LOC5564544 ELISA Kit
    Background
    Alternative name: ErbB-1, ErbB1, ER1, ERB-B1, MENA, HER1, Erythroblastic Leukemia Viral(v-Erb-B)oncogene Homolog,Avian, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1
    Gene ID
    24329
    UniProt
    Q9QX70
    Pathways
    NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins
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