Interferon gamma ELISA Kit

Catalog No. ABIN1672731

This Rat Interferon gamma ELISA Kit is a Colorimetric ELISA Kit designed to quantify Rat Interferon gamma. This ELISA Kit has been cited in 8+ publications.

Quick Overview for Interferon gamma ELISA Kit (ABIN1672731)

Target: Interferon gamma (IFNG)

Binding Specificity: AA 23-156

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2-2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum

Product Details Interferon gamma ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat IFN gamma

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli
Immunogen sequence: E23-C156

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <6pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: E23-C156

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Tissue Specificity: Released primarily from activated T lymphocytes.

Plate: Pre-coated

Protocol: rat IFN gamma ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IFN gamma has been precoated onto 96-well plates. Standards(E.coli, E23-C156) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IFN gamma is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat IFN gamma amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, rat IFN gamma standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each rat IFN gamma standard solution and each sample is measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 112, Standard deviation: 5.71, CV(%): 5.1
• Sample 2: n=16, Mean(pg/ml): 763, Standard deviation: 24.42, CV(%): 3.2
• Sample 3: n=16, Mean(pg/ml): 1479, Standard deviation: 42.89, CV(%): 2.9,
• Sample 1: n=24, Mean(pg/ml): 105, Standard deviation: 9.03, CV(%): 8.6
• Sample 2: n=24, Mean(pg/ml): 746, Standard deviation: 55.95, CV(%): 7.5
• Sample 3: n=24, Mean(pg/ml): 1389, Standard deviation: 79.17, CV(%): 5.7

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for Interferon gamma ELISA Kit (ABIN1672731)

Chen, Wu, Chen, Xie, Fang, Hu, Chen, Fu, He: "Omega-3 polyunsaturated fatty acid supplementation attenuates microglial-induced inflammation by inhibiting the HMGB1/TLR4/NF-κB pathway following experimental traumatic brain injury." in: Journal of neuroinflammation, Vol. 14, Issue 1, pp. 143, (2018) (PubMed).

Chen, Ye, Chen, Shi, Wu, Lin, Lin, Li, Fu, Li: "Valproic acid attenuates traumatic spinal cord injury-induced inflammation via STAT1 and NF-κB pathway dependent of HDAC3." in: Journal of neuroinflammation, Vol. 15, Issue 1, pp. 150, (2018) (PubMed).

Fu, Wang, Cai, Zhao, Fu: "Theaflavin alleviates inflammatory response and brain injury induced by cerebral hemorrhage via inhibiting the nuclear transcription factor kappa β-related pathway in rats." in: Drug design, development and therapy, Vol. 12, pp. 1609-1619, (2018) (PubMed).

Bai, Yang, Zhou, Cai, Buist-Homan, Cheng, Jiang, Shen, Li, Luo, Faber, Moshage, Shi: "The protective effect of the natural compound hesperetin against fulminant hepatitis in vivo and in vitro." in: British journal of pharmacology, Vol. 174, Issue 1, pp. 41-56, (2017) (PubMed).

Bi, Bi, Zhong, Zhang, Jin, Ma, Luo: "Effects of leukotriene B4 on interleukin-32, interferon-γ and chemokines in rats with rheumatoid arthritis." in: Experimental and therapeutic medicine, Vol. 14, Issue 4, pp. 2925-2930, (2017) (PubMed).

Hu, Wang, Zhang, Peng, Su, Chang, Liu: "Inhibition of myeloid differentiation factor 88 signaling mediated by histidine-grafted poly(β-amino ester) ester nanovector induces donor-specific liver allograft tolerance." in: International journal of nanomedicine, Vol. 10, pp. 4367-82, (2016) (PubMed).

Pan, Wang: "Synergistic interaction between choline and aspirin against acute inflammation induced by carrageenan and lipopolysaccharide." in: International immunopharmacology, Vol. 20, Issue 1, pp. 229-37, (2014) (PubMed).

Sofian, Aghakhani, Farazi, Banifazl, Eslamifar, Rashidi, Khadem Sadegh, Ramezani: "Serum profile of T helper 1 and T helper 2 cytokines in hepatitis C virus infected patients." in: Hepatitis monthly, Vol. 12, Issue 12, pp. e6156, (2013) (PubMed).

Target Details for Interferon gamma

Target: Interferon gamma (IFNG)

Alternative Name: IFNG

Background:

Protein Function: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

Background: Interferon-gamma(IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer(NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.

Synonyms: Interferon gamma,IFN-gamma,Ifng,

Full Gene Name: Interferon gamma

Cellular Localisation: Secreted.

Gene ID: 25712

UniProt: P01581

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy