GDF15 ELISA Kit

Catalog No. ABIN1672794

This Human GDF15 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human GDF15.

Quick Overview for GDF15 ELISA Kit (ABIN1672794)

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Binding Specificity: AA 197-308

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2-2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Product Details GDF15 ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human GDF-15

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: CHO
Immunogen sequence: A197-I308

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: CHO
Immunogen sequence: A197-I308

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Belongs to the TGF-beta family.

Tissue Specificity: Highly expressed in placenta, with lower levels in prostate and colon and some expression in kidney.

Plate: Pre-coated

Protocol: human GDF-15 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for GDF-15 has been precoated onto 96-well plates. Standards(CHO, A197-I308) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GDF-15 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human GDF-15 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human GDF-15 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human GDF-15 standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 104, Standard deviation: 2.5, CV(%): 2.4
• Sample 2: n=16, Mean(pg/ml): 735, Standard deviation: 27.93, CV(%): 3.8
• Sample 3: n=16, Mean(pg/ml): 1518, Standard deviation: 68.31, CV(%): 4.5,
• Sample 1: n=24, Mean(pg/ml): 121, Standard deviation: 6.897, CV(%): 5.7
• Sample 2: n=24, Mean(pg/ml): 757, Standard deviation: 46.18, CV(%): 6.1
• Sample 3: n=24, Mean(pg/ml): 1526, Standard deviation: 112.9, CV(%): 7.4

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for GDF15

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Alternative Name: GDF15

Background:

Background: GDF-15(Growth differentiation factor 15),also known as TGF-PL, MIC-1, PDF, PLAB, and PTGFB, is a protein belonging to the transforming growth factor beta superfamily that has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes. Using FISH, the MIC1 gene is mapped to 19p13.2-p13.1. Its expression in liver can be significantly up-regulated in during injury of organs such as liver, kidney, heart and lung. GDF15 showed increased expression and secretion during erythroblast maturation. GDF15 functions as an antiinflammatory cytokine by directly interfering with chemokine signaling and integrin activation.

Synonyms: Growth/differentiation factor 15,GDF-15,Macrophage inhibitory cytokine 1,MIC-1,NSAID-activated gene 1 protein,NAG-1,NSAID-regulated gene 1 protein,NRG-1,Placental TGF-beta,Placental bone morphogenetic protein,Prostate differentiation factor,GDF15,MIC1, PDF, PLAB, PTGFB,

Full Gene Name: Growth/differentiation factor 15

Cellular Localisation: Secreted.

Gene ID: 9518

UniProt: Q99988

Pathways: SARS-CoV-2 Protein Interactome