GDF15 ELISA Kit

Catalog No. ABIN1706082

Product Details GDF15 ELISA Kit

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Binding Specificity: AA 189-303

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6-1000 pg/mL

Minimum Detection Limit: 15.6 pg/mL

Application: ELISA

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse GDF-15

Brand: PicoKine™

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: S189-A303

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: S189-A303

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Tissue Specificity: Highly expressed in liver. .

Plate: Pre-coated

Protocol: mouse GDF-15 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for GDF-15 has been precoated onto 96-well plates. Standards(NSO, S189-A303) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GDF-15 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse GDF-15 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL mouse GDF-15 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse GDF-15 standard solution and each sample is measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 114, Standard deviation: 4.33, CV(%): 3.8
• Sample 2: n=16, Mean(pg/ml): 346, Standard deviation: 12.46, CV(%): 3.6
• Sample 3: n=16, Mean(pg/ml): 548, Standard deviation: 22.47, CV(%): 4.1,
• Sample 1: n=24, Mean(pg/ml): 98.2, Standard deviation: 7.37, CV(%): 7.5
• Sample 2: n=24, Mean(pg/ml): 359, Standard deviation: 20.10, CV(%): 5.6
• Sample 3: n=24, Mean(pg/ml): 579, Standard deviation: 39.37, CV(%): 6.8

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for GDF15

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Alternative Name: GDF15 (GDF15 Products)

Synonyms: GDF15 ELISA Kit, GDF-15 ELISA Kit, MIC-1 ELISA Kit, MIC1 ELISA Kit, NAG-1 ELISA Kit, PDF ELISA Kit, PLAB ELISA Kit, PTGFB ELISA Kit, SBF ELISA Kit, growth differentiation factor 15 ELISA Kit, GDF15 ELISA Kit, Gdf15 ELISA Kit

Background:

Background: GDF-15(Growth differentiation factor 15),also known as TGF-PL, MIC-1, PDF, PLAB, and PTGFB, is a protein belonging to the transforming growth factor beta superfamily that has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes. Using FISH, the MIC1 gene is mapped to 19p13.2-p13.1. Its expression in liver can be significantly up-regulated in during injury of organs such as liver, kidney, heart and lung. GDF15 showed increased expression and secretion during erythroblast maturation. GDF15 functions as an antiinflammatory cytokine by directly interfering with chemokine signaling and integrin activation.

Synonyms: Growth/differentiation factor 15,GDF-15,Gdf15,Sbf,

Full Gene Name: Growth/differentiation factor 15

Cellular Localisation: Secreted.

Gene ID: 23886

UniProt: Q9Z0J7

Pathways: SARS-CoV-2 Protein Interactome