CCL2 ELISA Kit

Catalog No. ABIN1672827

The Rat CCL2 ELISA Kit (ABIN1672827) is a Colorimetric ELISA Kit designed to quantify Rat CCL2. This ELISA Kit has been cited in 23 publications.

Quick Overview for CCL2 ELISA Kit (ABIN1672827)

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Binding Specificity: AA 24-148

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6 pg/mL - 1000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum

Product Details CCL2 ELISA Kit

Minimum Detection Limit: 15.6 pg/mL

Purpose: Sandwich Rat MCP-1 ELISA Kit to quantitate Rat CCL2 in cell culture supernatants and serum.

Analytical Method: Quantitative

Specificity: Expression system for standard: NS0
Immunogen sequence: Q24-N148

Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 7.2%, 7.2%, 5.8%
Inter-Assay Precision (CV%): 6.4%, 8.6%, 5.8%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles. (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

References for CCL2 ELISA Kit (ABIN1672827)

Zhang, Xu, Hu, Wu, Qin, Zhang: "Anti-inflammatory effects of Lefty-1 in renal tubulointerstitial inflammation via regulation of the NF-κB pathway." in: International journal of molecular medicine, Vol. 41, Issue 3, pp. 1293-1304, (2018) (PubMed).

Mei, Wang, Li, Liu, Lu, Li, Zhang, Tian: "Dusuqing granules (DSQ) suppress inflammation in Klebsiella pneumonia rat via NF-κB/MAPK signaling." in: BMC complementary and alternative medicine, Vol. 17, Issue 1, pp. 216, (2017) (PubMed).

Liu, Zhang, Han, Wang, Liu, Zhang, Zhou, Xiang: "Inhibition of BTK protects lungs from trauma-hemorrhagic shock-induced injury in rats." in: Molecular medicine reports, Vol. 16, Issue 1, pp. 192-200, (2017) (PubMed).

Bi, Bi, Zhong, Zhang, Jin, Ma, Luo: "Effects of leukotriene B4 on interleukin-32, interferon-γ and chemokines in rats with rheumatoid arthritis." in: Experimental and therapeutic medicine, Vol. 14, Issue 4, pp. 2925-2930, (2017) (PubMed).

Yang, Zhang, Wang, Zhao, Shi: "SOCS2 overexpression alleviates diabetic nephropathy in rats by inhibiting the TLR4/NF-κB pathway." in: Oncotarget, Vol. 8, Issue 53, pp. 91185-91198, (2017) (PubMed).

Chen, Zhang, Ma, Huang, Shao, Wu, Wang: "Naringin Alleviates Diabetic Kidney Disease through Inhibiting Oxidative Stress and Inflammatory Reaction." in: PLoS ONE, Vol. 10, Issue 11, pp. e0143868, (2016) (PubMed).

Mao, Sun, Mao, Wang, Zhang, Zhou, Rahman, Ye: "Inhibitory Effects of Angelica Polysaccharide on Activation of Mast Cells." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2016, pp. 6063475, (2016) (PubMed).

Tu, Chen, Yang, Qi, Lu, Zhao: "Amyloid-? Activates Microglia and Regulates Protein Expression in a Manner Similar to Prions." in: Journal of molecular neuroscience : MN, Vol. 56, Issue 2, pp. 509-18, (2015) (PubMed).

Jiang, Zhang, Xiong, Jia, Sun, Lin, Shen, Xie, Yan: "EETs Attenuate Ox-LDL-Induced LTB4 Production and Activity by Inhibiting p38 MAPK Phosphorylation and 5-LO/BLT1 Receptor Expression in Rat Pulmonary Arterial Endothelial Cells." in: PLoS ONE, Vol. 10, Issue 6, pp. e0128278, (2015) (PubMed).

Aizman, Vinodkumar, McGrogan, Bates: "Cell Injury-Induced Release of Fibroblast Growth Factor 2: Relevance to Intracerebral Mesenchymal Stromal Cell Transplantations." in: Stem cells and development, Vol. 24, Issue 14, pp. 1623-34, (2015) (PubMed).

Dai, Wu, He, Zhang, Chen, Xu, Wang, Gruzdev, Zeldin, Wang: "Epoxyeicosatrienoic acids regulate macrophage polarization and prevent LPS-induced cardiac dysfunction." in: Journal of cellular physiology, Vol. 230, Issue 9, pp. 2108-19, (2015) (PubMed).

Zhang, Jiang, Chen, Wang, Feng, Tao, Qin: "Serum Levels of IL-1 ? , IL-6, TGF- ? , and MMP-9 in Patients Undergoing Carotid Artery Stenting and Regulation of MMP-9 in a New In Vitro Model of THP-1 Cells Activated by Stenting." in: Mediators of inflammation, Vol. 2015, pp. 956082, (2015) (PubMed).

Wei, Zhang, He, Wang, Liu, Liu, Sun, Zhou, Bai, Yuan et al.: "Protective effect of saponins extract from Panax japonicus on myocardial infarction: involvement of NF-?B, Sirt1 and mitogen-activated protein kinase signalling pathways and inhibition of ..." in: The Journal of pharmacy and pharmacology, Vol. 66, Issue 11, pp. 1641-51, (2014) (PubMed).

Tu, Yang, Zhou, Qi, Wang, Kouadir, Xu, Yin, Zhao: "PrP106-126 and A? 1-42 peptides induce BV-2 microglia chemotaxis and proliferation." in: Journal of molecular neuroscience : MN, Vol. 52, Issue 1, pp. 107-16, (2014) (PubMed).

Wang, Gao, Wang, Zhao, Nie, Shi, Zhao, Lu, Wang, Yang: "Green tea polyphenol epigallocatechin-3-gallate inhibits TNF-?-induced production of monocyte chemoattractant protein-1 in human umbilical vein endothelial cells." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 33, Issue 5, pp. 1349-58, (2014) (PubMed).

Meneses, Libório, de Daher, da Silva, da Costa, Pontes, Martins: "Urinary monocyte chemotactic protein-1 (MCP-1) in leprosy patients: increased risk for kidney damage." in: BMC infectious diseases, Vol. 14, pp. 451, (2014) (PubMed).

Xu, Kong, Zhou, Zhang, Liu, Yan, Xie, Xie: "oxLDL/?2GPI/anti-?2GPI complex induced macrophage differentiation to foam cell involving TLR4/NF-kappa B signal transduction pathway." in: Thrombosis research, Vol. 134, Issue 2, pp. 384-92, (2014) (PubMed).

Lin, Liu, Fang, Yu, Xiong, Yuan: "Anti-fatigue and vasoprotective effects of quercetin-3-O-gentiobiose on oxidative stress and vascular endothelial dysfunction induced by endurance swimming in rats." in: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Vol. 68, pp. 290-6, (2014) (PubMed).

Huang, Wang, Liu, Zheng, Li: "Inhibitory effect of Malvidin on TNF-?-induced inflammatory response in endothelial cells." in: European journal of pharmacology, Vol. 723, pp. 67-72, (2014) (PubMed).

Jiang, Zhang, Liu, Lin, Sun, Shen, Yan, Xie: "EETs alleviate ox-LDL-induced inflammation by inhibiting LOX-1 receptor expression in rat pulmonary arterial endothelial cells." in: European journal of pharmacology, Vol. 727, pp. 43-51, (2014) (PubMed).

Target Details for CCL2

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Alternative Name: CCL2

Background:

Background: Monocyte chemoattractant protein-1 (MCP-1), a member of the chemokine (chemotactic cytokine) family, is a potent monocyte agonist that is upregulated by oxidized lipids.1 MCP-1 is also known as CCL2, SCYA2, MCAF. MCAF is a member of family of factors involved in immune and inflammatory responses. The amino acid sequence deduced from the nucleotide sequence reveals the primary structure of the MCAF precursor to be composed of a putative signal peptide sequence of 23 amino acid residues and a mature MCAF sequence of 76 amino acid residues.2 MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder.3

Gene Full Name: C-C motif chemokine ligand 2

Gene ID: 24770

UniProt: P14844

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, The Global Phosphorylation Landscape of SARS-CoV-2 Infection