LYVE1 ELISA Kit

Catalog No. ABIN1672871

This Colorimetric ELISA kit is designed for the quantitative measurement of Human LYVE1. There is 1 publication available.

Quick Overview for LYVE1 ELISA Kit (ABIN1672871)

Target: LYVE1 (Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE1))

Binding Specificity: AA 24-238

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate

Product Details LYVE1 ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Purpose: Sandwich Human LYVE-1 ELISA Kit to quantitate Human LYVE1 in cell culture supernatants and cell lysates.

Analytical Method: Quantitative

Specificity: Expression system for standard: NS0
Immunogen sequence: S24-T238

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 6.7%, 6.7%, 5.3%
Inter-Assay Precision (CV%): 7.5%, 6.9%, 7.1%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

References for LYVE1 ELISA Kit (ABIN1672871)

Du, Chen, Wang, Wen, Wang, Wang, Kan, Wei, Zhao: "VEGF-D-induced draining lymphatic enlargement and tumor lymphangiogenesis promote lymph node metastasis in a xenograft model of ovarian carcinoma." in: Reproductive biology and endocrinology : RB&E, Vol. 12, pp. 14, (2014) (PubMed).

Target Details for LYVE1

Target: LYVE1 (Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE1))

Alternative Name: LYVE1

Background:

Background: Extracellular link domain containing 1, also known as LYVE-1 or XLKD1 is a human gene. The International Radiation Hybrid Mapping Consortium mapped the LYVE1 gene to chromosome 11p15.4. This gene encodes a type I integral membrane glycoprotein. The encoded protein acts as a receptor and binds to both soluble and immobilized hyaluronan. This protein may function in lymphatic hyaluronan transport and have a role in tumor metastasis. This gene may play a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). It may act as a hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes.

Gene Full Name: lymphatic vessel endothelial hyaluronan receptor 1

Gene ID: 10894

UniProt: Q9Y5Y7

Pathways: Glycosaminoglycan Metabolic Process