SensoLyte® Plus 520 MMP-2 Assay Kit Fluorimetric and Enhanced Selectivity
Quick Overview for SensoLyte® Plus 520 MMP-2 Assay Kit Fluorimetric and Enhanced Selectivity (ABIN1882520)
Target
See all MMP2 KitsReactivity
Detection Method
Application
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Characteristics
- The SensoLyte® Plus 520 MMP-2 Assay Kit is designed specifically for detecting MMP-2 activity in biological samples, such as culture medium, serum, plasma, synovial fluid, and tissue homogenate, which may contain multiple MMPs. Members of the MMP family have poor substrate sequence specificity, thus using peptide substrate alone to differentiate the activity of a particular MMP from other MMPs is inadequate. A monoclonal anti-human-MMP-2 antibody is therefore employed to pull down MMP-2 from the mixture. MMP-2 activity is then quantified by a 5-FAM/QXL™ 520 fluorescence resonance energy transfer (FRET) peptide (Scheme 1). The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds. The assay can detect as low as 3 ng/mL active MMP-2 enzyme. The assay has no cross-reactivity with human MMP-1, 3, 7, 8, 9, 10, 12, 13, 15, 16 and has minimal cross-reactivity with MMP-14).
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Matrix Metalloproteinase 2 (MMP2) ELISA Kit
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SensoLyte® 520 MMP-2 Assay KitFRET Fluorometric
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Comment
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ELISA + FRET-based Assay Kit
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Restrictions
- For Research Use only
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Storage
- -80 °C
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Storage Comment
- Store all kit components, except Component B, at -20 °C. Store Component B at -80 °C. For convenience, Components D, G, H can be stored at RT.
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- MMP2 (Matrix Metalloproteinase 2 (MMP2))
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Alternative Name
- MMP-2
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Background
- Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated zinc endopeptidases capable of digesting extracellular matrix components. MMP-2 (72 kDa gelatinase-A/type IV collagenase) is responsible for degradation of collagen, fibronectin, laminin, and elastin. It has been shown that MMP-2 plays a key role in angiogenesis, tumor cell invasion, and metastasis. MMP-2 is also considered a therapeutic target for cancer.
Target
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