SensoLyte® Plus 520 MMP-2 Assay Kit Fluorimetric and Enhanced Selectivity

Catalog No. ABIN1882520

Product Details

Target: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Reactivity: Human

Detection Method: Fluorometric

Application: Fluorescence Resonance Energy Transfer Microscopy (FRET)

Brand: SensoLyte®

Characteristics: The SensoLyte® Plus 520 MMP-2 Assay Kit is designed specifically for detecting MMP-2 activity in biological samples, such as culture medium, serum, plasma, synovial fluid, and tissue homogenate, which may contain multiple MMPs. Members of the MMP family have poor substrate sequence specificity, thus using peptide substrate alone to differentiate the activity of a particular MMP from other MMPs is inadequate. A monoclonal anti-human-MMP-2 antibody is therefore employed to pull down MMP-2 from the mixture. MMP-2 activity is then quantified by a 5-FAM/QXL™ 520 fluorescence resonance energy transfer (FRET) peptide (Scheme 1). The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds. The assay can detect as low as 3 ng/mL active MMP-2 enzyme. The assay has no cross-reactivity with human MMP-1, 3, 7, 8, 9, 10, 12, 13, 15, 16 and has minimal cross-reactivity with MMP-14).

Application Details

Comment:

ELISA + FRET-based Assay Kit

Restrictions: For Research Use only

Handling

Storage: -80 °C

Storage Comment: Store all kit components, except Component B, at -20 °C. Store Component B at -80 °C. For convenience, Components D, G, H can be stored at RT.

Target Details

Target: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Alternative Name: MMP-2 (MMP2 Products)

Synonyms: 2-MMP Kit, CG1794 Kit, DM2-MMP Kit, Dm2-MMP Kit, Dmel\\CG1794 Kit, MMP2 Kit, anon-WO0118547.84 Kit, dm-2MMP Kit, dmmp2 Kit, l(2)02353 Kit, mmp2 Kit, wu:fa99h12 Kit, wu:fk89d01 Kit, fgmmp-2 Kit, Mmp-2 Kit, LOC100135793 Kit, Clg4a Kit, GelA Kit, MMP-2 Kit, CLG4 Kit, CLG4A Kit, MMP-II Kit, MONA Kit, TBE-1 Kit, matrix metallopeptidase 2 Kit, Matrix metalloproteinase 2 Kit, matrix metalloproteinase 2 Kit, matrix metalloproteinase-2 Kit, MMP2 Kit, Mmp2 Kit, mmp2 Kit, LOC657982 Kit, LOC100135793 Kit

Background: Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated zinc endopeptidases capable of digesting extracellular matrix components. MMP-2 (72 kDa gelatinase-A/type IV collagenase) is responsible for degradation of collagen, fibronectin, laminin, and elastin. It has been shown that MMP-2 plays a key role in angiogenesis, tumor cell invasion, and metastasis. MMP-2 is also considered a therapeutic target for cancer.