ESM1 ELISA Kit

Catalog No. ABIN1889317

Human ESM1 ELISA Kit, Colorimetric assay for quantification of Human ESM1, that has been mentioned in 3+ publications.

Quick Overview for ESM1 ELISA Kit (ABIN1889317)

Target: ESM1 (Endothelial Cell-Specific Molecule 1 (ESM1))

Binding Specificity: AA 20-184

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Product Details ESM1 ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Purpose: Sandwich Human ESM1/Endocan ELISA Kit to quantitate Human ESM1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Analytical Method: Quantitative

Specificity: Expression system for standard: NS0
Immunogen sequence: W20-R184

Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 7.9%, 5.5%, 5.5%
Inter-Assay Precision (CV%): 8.8%, 6.6%, 6.3%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

References for ESM1 ELISA Kit (ABIN1889317)

Sabry, Sabry, Daifalla, Akl, Hamed, Torky: "Serum markers for asymptomatic atherosclerosis in Egyptian psoriatic patients: study controlled by Doppler estimation of carotid intima-media thickness." in: Vascular health and risk management, Vol. 14, pp. 145-152, (2018) (PubMed).

Lee, Kim, Kim, Kim, Moon, Jeong, Lee, Ihm, Lee: "Plasma endocan level and prognosis of immunoglobulin A nephropathy." in: Kidney research and clinical practice, Vol. 35, Issue 3, pp. 152-9, (2016) (PubMed).

Çimen, Bilgin, Akyel, Felekoğlu, Nallbani, Özdemir, Erden, Öztürk, Doğan, Yeter: "Endocan and Non-Dipping Circadian Pattern in Newly Diagnosed Essential Hypertension." in: Korean circulation journal, Vol. 46, Issue 6, pp. 827-833, (2016) (PubMed).

Target Details for ESM1

Target: ESM1 (Endothelial Cell-Specific Molecule 1 (ESM1))

Alternative Name: ESM1

Background:

Background: Endothelial cell-specific molecule 1, also known as Endocan, is a protein that in humans is encoded by the ESM1 gene. This gene encodes a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability signals. ESM1 has been described as a specific biomarker of tip cells during neoangiogenesis by independent teams. Its expression has been shown to be increase in presence of pro-angiogenic growth factors such as VEGF (vascular endothelial growth factor) or FGF-2 (fibroblast growth factor 2).

Gene Full Name: endothelial cell specific molecule 1

Gene ID: 11082

UniProt: Q9NQ30

Pathways: Growth Factor Binding