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ESM1 ELISA Kit

ESM1 Reactivity: Mouse Colorimetric Sandwich ELISA 23.4 pg/mL - 1500 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Catalog No. ABIN6720142
  • Target See all ESM1 ELISA Kits
    ESM1 (Endothelial Cell-Specific Molecule 1 (ESM1))
    Reactivity
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    23.4 pg/mL - 1500 pg/mL
    Minimum Detection Limit
    23.4 pg/mL
    Application
    ELISA
    Purpose
    Sandwich ELISA for Quantitative Detection of Antigen
    Sample Type
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytical Method
    Quantitative
    Characteristics

    Synonyms: Endothelial cell-specific molecule 1, ESM-1, Esm1, ESM1_HUMAN

    Background: Endothelial cell-specific molecule 1, also known as Endocan, is a protein that in humans is encoded by the ESM1 gene. This gene encodes a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability signals. ESM-1 has been described as a specific biomarker of tip cells during neoangiogenesis by independent teams. Its expression has been shown to be increase in presence of pro-angiogenic growth factors such as VEGF(vascular endothelial growth factor) or FGF-2(fibroblast growth factor 2).

    Gene Name: ESM1

    Production: Natural and recombinant mouse ESM1. There is no detectable cross-reactivity with other relevant proteins.

    Standard: Expression system for standard: NSO, Immunogen sequence: W20-R184

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  • Application Notes

    Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1500pg/mL, 750pg/mL, 419pg/mL, 187.5pg/mL, 93.7pg/mL, 46.8pg/mL, 23.4pg/mL mouse ESM1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse ESM1 standard solution and each sample be measured in duplicate.

    Blood Product Anticoagulant: Heparin Sodium

    ELISA Dilution: 23.4pg/mL-1500pg/mL

    Sample Volume
    100 μL
    Plate
    Pre-coated
    Restrictions
    For Research Use only
  • Storage
    RT,4 °C,-20 °C
    Storage Comment
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
  • Target See all ESM1 ELISA Kits
    ESM1 (Endothelial Cell-Specific Molecule 1 (ESM1))
    Alternative Name
    ESM1 - Endocan (ESM1 Products)
    Synonyms
    ESM1 ELISA Kit, zgc:153027 ELISA Kit, 0610042H23Rik ELISA Kit, AV004503 ELISA Kit, ESM-1 ELISA Kit, Pg25 ELISA Kit, endocan ELISA Kit, endothelial cell specific molecule 1 ELISA Kit, endothelial cell-specific molecule 1 ELISA Kit, ESM1 ELISA Kit, esm1 ELISA Kit, Esm1 ELISA Kit
    Gene ID
    71690
    NCBI Accession
    NP_076101
    UniProt
    Q9QYY7
    Pathways
    Growth Factor Binding
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