GAS6 ELISA Kit

Catalog No. ABIN1889367

This Mouse GAS6 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Mouse GAS6.

Quick Overview for GAS6 ELISA Kit (ABIN1889367)

Target: GAS6 (Growth Arrest-Specific 6 (GAS6))

Binding Specificity: AA 115-673

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2-2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Product Details GAS6 ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse GAS6

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: D115-P673

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: D115-P673

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Contains 4 EGF-like domains.

Plate: Pre-coated

Protocol: mouse GAS6 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for GAS6 has been precoated onto 96-well plates. Standards(NSO, D115-P673) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GAS6 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse GAS6 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse GAS6 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse GAS6 standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 156, Standard deviation: 9.048, CV(%): 5.8
• Sample 2: n=16, Mean(pg/ml): 724, Standard deviation: 34.03, CV(%): 4.7
• Sample 3: n=16, Mean(pg/ml): 1345, Standard deviation: 55.15, CV(%): 4.1,
• Sample 1: n=24, Mean(pg/ml): 174 , Standard deviation: 11.31, CV(%): 6.5
• Sample 2: n=24, Mean(pg/ml): 617, Standard deviation: 33.32, CV(%): 5.4
• Sample 3: n=24, Mean(pg/ml): 1436, Standard deviation: 112.01, CV(%): 7.8

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for GAS6

Target: GAS6 (Growth Arrest-Specific 6 (GAS6))

Alternative Name: GAS6

Background:

Protein Function: Ligand for tyrosine-protein kinase receptors AXL, TYRO3 and MER whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. .

Background: GAS6(Growth arrest-specific 6), also known as AXLLG or AXSF, is a human gene coding for the Gas6 protein. It is mapped to 13q34. GAS6 was originally found as a gene upregulated by growth arrested fibroblasts. This gene product is a gamma-carboxyglutamic acid(Gla)-containing protein which thought to be involved in the stimulation of cell proliferation, and may play a role in thrombosis. What's more, the product of the GAS6 gene, a ligand for the AXL, MER, and TYRO3 tyrosine kinase receptors, is a vitamin K-dependent protein, structurally related to anticoagulant protein S but lacking its anticoagulant activity. This protein also shows great importance in the cardiovascular system.

Synonyms: Growth arrest-specific protein 6,GAS-6,AXL receptor tyrosine kinase ligand,Gas6,

Full Gene Name: Growth arrest-specific protein 6

Cellular Localisation: Secreted.

Gene ID: 14456

UniProt: Q61592

Pathways: RTK Signaling, Carbohydrate Homeostasis, Production of Molecular Mediator of Immune Response