TGFBI ELISA Kit

Catalog No. ABIN1889371

Human TGFBI ELISA Kit Colorimetric assay for quantification of Human TGFBI and has been mentioned in 1 publication.

Quick Overview for TGFBI ELISA Kit (ABIN1889371)

Target: TGFBI (Transforming Growth Factor, beta-Induced, 68kDa (TGFBI))

Binding Specificity: AA 24-683

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5-4000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Product Details TGFBI ELISA Kit

Minimum Detection Limit: 62.5 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TGFBI/betaIG-H3

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: G24-H683

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: G24-H683

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Contains 1 EMI domain.

Tissue Specificity: Highly expressed in the corneal epithelium. .

Plate: Pre-coated

Protocol: human TGFBI ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TGFBI has been precoated onto 96-well plates. Standards(NSO, G24-H683) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TGFBI is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TGFBI amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human TGFBI standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TGFBI standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 672, Standard deviation: 30.24, CV(%): 4.5
• Sample 2: n=16, Mean(pg/ml): 1913, Standard deviation: 89.9, CV(%): 4.7
• Sample 3: n=16, Mean(pg/ml): 2844, Standard deviation: 150.7, CV(%): 5.3,
• Sample 1: n=24, Mean(pg/ml): 764, Standard deviation: 39.7, CV(%): 5.2
• Sample 2: n=24, Mean(pg/ml): 1860, Standard deviation: 106.02, CV(%): 5.7
• Sample 3: n=24, Mean(pg/ml): 2927, Standard deviation: 184.4, CV(%): 6.3

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for TGFBI ELISA Kit (ABIN1889371)

Jin, Wan, Chen, Chen, Zhang, Deng, Zhang, Xiong, Xin: "Treg/IL-17 ratio and Treg differentiation in patients with COPD." in: PLoS ONE, Vol. 9, Issue 10, pp. e111044, (2014) (PubMed).

Target Details for TGFBI

Target: TGFBI (Transforming Growth Factor, beta-Induced, 68kDa (TGFBI))

Alternative Name: TGFBI

Background:

Protein Function: Binds to type I, II, and IV collagens. This adhesion protein may play an important role in cell-collagen interactions. In cartilage, may be involved in endochondral bone formation.

Background: Transforming growth factor, beta-induced, 68 kDa, also known as TGFBI(initially called BIGH3, BIG-H3), is a protein which in humans is encoded by the TGFBI gene. It is mapped to 5q31.1. This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion.

Synonyms: Transforming growth factor-beta-induced protein ig-h3,Beta ig-h3,Kerato-epithelin,RGD-containing collagen-associated protein,RGD-CAP,TGFBI,BIGH3,

Full Gene Name: Transforming growth factor-beta-induced protein ig-h3

Cellular Localisation: Secreted, extracellular space, extracellular matrix. May be associated both with microfibrils and with the cell surface.

Gene ID: 7045

UniProt: Q15582