Trefoil Factor 2 ELISA Kit

Catalog No. ABIN1889395

Product Details Trefoil Factor 2 ELISA Kit

Target: Trefoil Factor 2 (TFF2)

Binding Specificity: AA 24-129

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 7.8-500 pg/mL

Minimum Detection Limit: 7.8 pg/mL

Application: ELISA

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TFF2

Brand: PicoKine™

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: E24-Y129

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: E24-Y129

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Contains 2 P-type (trefoil) domains.

Tissue Specificity: Stomach.

Plate: Pre-coated

Protocol: human TFF2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TFF2 has been precoated onto 96-well plates. Standards(NSO, E24-Y129) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TFF2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TFF2 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL human TFF2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TFF2 standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 62, Standard deviation: 2.3, CV(%): 3.7
• Sample 2: n=16, Mean(pg/ml): 126, Standard deviation: 5.3, CV(%): 4.2
• Sample 3: n=16, Mean(pg/ml): 255, Standard deviation: 13.77, CV(%): 5.4,
• Sample 1: n=24, Mean(pg/ml): 58, Standard deviation: 2.67, CV(%): 4.6
• Sample 2: n=24, Mean(pg/ml): 133, Standard deviation: 7.85, CV(%): 5.9
• Sample 3: n=24, Mean(pg/ml): 263, Standard deviation: 17.62, CV(%): 6.7

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for Trefoil Factor 2

Target: Trefoil Factor 2 (TFF2)

Alternative Name: TFF2 (TFF2 Products)

Synonyms: TFF2 ELISA Kit, SML1 ELISA Kit, SP ELISA Kit, PSP ELISA Kit, p4 ELISA Kit, p4-a ELISA Kit, sml1 ELISA Kit, xP4 ELISA Kit, xp4.1 ELISA Kit, mSP ELISA Kit, trefoil factor 2 ELISA Kit, trefoil factor 2 S homeolog ELISA Kit, trefoil factor 2 (spasmolytic protein 1) ELISA Kit, TFF2 ELISA Kit, Tff2 ELISA Kit, tff2.S ELISA Kit

Background:

Protein Function: Inhibits gastrointestinal motility and gastric acid secretion. Could function as a structural component of gastric mucus, possibly by stabilizing glycoproteins in the mucus gel through interactions with carbohydrate side chains (By similarity). .

Background: Trefoil factor 2, also known as SP or SML1, is a protein that in humans is encoded by the TFF2 gene. This gene belongs to trefoil family, and members of the trefoil family may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. This gene is mapped to 21q22.3. TFF2 gene consists of 4 exons spanning 5.1 kb, with exons 2 and 3 encoding 1 trefoil domain each, and it is the only gene encoding 2 TFF-domains on separate exons. It has been found that the distribution of TFF2 and pS2 suggests involvement in repair-enhancing mechanisms.

Synonyms: Trefoil factor 2,Spasmolysin,Spasmolytic polypeptide,SP,TFF2,SML1,

Full Gene Name: Trefoil factor 2

Cellular Localisation: Secreted.

Gene ID: 7032

UniProt: Q03403