Trefoil Factor 2 ELISA Kit
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- Target See all Trefoil Factor 2 (TFF2) ELISA Kits
- Trefoil Factor 2 (TFF2)
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 7.8 pg/mL - 500 pg/mL
- Minimum Detection Limit
- 7.8 pg/mL
- Application
- ELISA
- Purpose
- Sandwich ELISA for Quantitative Detection of Antigen
- Sample Type
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- Characteristics
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Synonyms: SML 1, SML1, SP, Spasmolysin, Spasmolytic polypeptide, spasmolytic protein 1, TFF 2, TFF2, TFF2_HUMAN, trefoil factor 2 (spasmolytic protein 1), Trefoil factor 2, Trefoil factor 2 precursor
Background: Trefoil factor 2, also known as SP or SML1, is a protein that in humans is encoded by the TFF2 gene. This gene belongs to trefoil family, and members of the trefoil family may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. This gene is mapped to 21q22.3. TFF2 gene consists of 4 exons spanning 5.1 kb, with exons 2 and 3 encoding 1 trefoil domain each, and it is the only gene encoding 2 TFF-domains on separate exons. It has been found that the distribution of TFF2 and pS2 suggests involvement in repair-enhancing mechanisms.
Gene Name: TFF2
Production: Natural and recombinant human TFF2. There is no detectable cross-reactivity with other relevant proteins.
Standard: Expression system for standard: NSO, Immunogen sequence: E24-Y129
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- Discover our top product TFF2 ELISA Kit
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- Application Notes
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Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL human TFF2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human TFF2 standard solution and each sample be measured in duplicate.
Blood Product Anticoagulant: Heparin Sodium
ELISA Dilution: 7.8pg/mL-500pg/mL
- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Restrictions
- For Research Use only
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- Storage
- RT,4 °C,-20 °C
- Storage Comment
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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- Target See all Trefoil Factor 2 (TFF2) ELISA Kits
- Trefoil Factor 2 (TFF2)
- Alternative Name
- TFF2 (TFF2 Products)
- Synonyms
- TFF2 ELISA Kit, SML1 ELISA Kit, SP ELISA Kit, PSP ELISA Kit, p4 ELISA Kit, p4-a ELISA Kit, sml1 ELISA Kit, xP4 ELISA Kit, xp4.1 ELISA Kit, mSP ELISA Kit, trefoil factor 2 ELISA Kit, trefoil factor 2 S homeolog ELISA Kit, trefoil factor 2 (spasmolytic protein 1) ELISA Kit, TFF2 ELISA Kit, Tff2 ELISA Kit, tff2.S ELISA Kit
- Gene ID
- 7032
- NCBI Accession
- NP_005414
- UniProt
- Q03403
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