CD25 ELISA Kit

Catalog No. ABIN2042394

This Mouse CD25 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Mouse CD25.

Quick Overview for CD25 ELISA Kit (ABIN2042394)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 8-5000 pg/mL

Application: ELISA

Product Details CD25 ELISA Kit

Minimum Detection Limit: 8 pg/mL

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 5 pg/mL

Components: 96-well plate pre-coated with anti-CD25/IL-2sR alpha, Mouse
Lyophilized recombinant CD25/IL-2sR alpha, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Application Details

Plate: Pre-coated

Protocol: Sandwich ELISA quantitative detection kit for Mouse CD25/IL-2sR alpha in cell culture supernates, serum and plasma (heparin, EDTA).CD25 is the alpha chain of the IL-2 receptor. It is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. CD25 has been used as a marker to identify CD4+FoxP3+ regulatory T cells in mice. By in situ hybridization, the IL2R gene was mapped to 10p15-p14. Its soluble form, called sIL-2R may be elevated in these diseases and is occasionally used to track disease progression.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Alternative Name: CD25/IL-2sR alpha

Gene ID: 16184

UniProt: P01590

Pathways: JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation