GDNF ELISA Kit
Quick Overview for GDNF ELISA Kit (ABIN2042483)
Target
See all GDNF ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 31.2 pg/mL
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Cross-Reactivity (Details)
- There is no detectable cross-reactivity with other relevant proteins.
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Sensitivity
- < 4 pg/mL
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Components
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96-well plate pre-coated with anti-GDNF, Rat
Lyophilized recombinant GDNF, Rat standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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Plate
- Pre-coated
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Protocol
- Sandwich ELISA quantitative detection kit for Rat GDNF in cell culture supernates, serum and plasma (heparin, EDTA).Glial cell line-derived neurotrophic factor (GDNF) is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily.1 GDNF, is a potent neurotrophic factor that promotes the survival of dopaminergic neurones in cultures including embryonic neuronal cultures.2 GDNF, in addition to its potential role in the differentiation and survival of central nervous system neurons, has profound effects on kidney organogenesis and the development of the peripheral nervous system.3 GDNF may have utility in the treatment of Parkinson's disease, which is marked by progressive degeneration of midbrain dopaminergic neurons.1 GDNF lies on the short arm of human chromosome 5, at 5p13.1-p13.3 ability to promote dopamine uptake in midbrain cultures.2 The standard product used in this kit is recombinant rat GDNF, which is a dimmer composed of two chains with 134 amino acids.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))
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Alternative Name
- GDNF
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Gene ID
- 25453
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UniProt
- A7UGJ1
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Pathways
- RTK Signaling, Synaptic Membrane, Tube Formation, Autophagy, Smooth Muscle Cell Migration
Target See all GDNF ELISA Kits
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