Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

GDNF ELISA Kit

This Colorimetric ELISA kit is designed for the quantitative measurement of Rat GDNF.
Catalog No. ABIN2042483

Quick Overview for GDNF ELISA Kit (ABIN2042483)

Target

See all GDNF ELISA Kits
GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Reactivity

  • 13
  • 10
  • 8
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Rat

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

31.2-2000 pg/mL

Application

ELISA
  • Minimum Detection Limit

    31.2 pg/mL

    Cross-Reactivity (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity

    < 4 pg/mL

    Components

    96-well plate pre-coated with anti-GDNF, Rat
    Lyophilized recombinant GDNF, Rat standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • Plate

    Pre-coated

    Protocol

    Sandwich ELISA quantitative detection kit for Rat GDNF in cell culture supernates, serum and plasma (heparin, EDTA).Glial cell line-derived neurotrophic factor (GDNF) is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily.1 GDNF, is a potent neurotrophic factor that promotes the survival of dopaminergic neurones in cultures including embryonic neuronal cultures.2 GDNF, in addition to its potential role in the differentiation and survival of central nervous system neurons, has profound effects on kidney organogenesis and the development of the peripheral nervous system.3 GDNF may have utility in the treatment of Parkinson's disease, which is marked by progressive degeneration of midbrain dopaminergic neurons.1 GDNF lies on the short arm of human chromosome 5, at 5p13.1-p13.3 ability to promote dopamine uptake in midbrain cultures.2 The standard product used in this kit is recombinant rat GDNF, which is a dimmer composed of two chains with 134 amino acids.

    Restrictions

    For Research Use only
  • Storage

    4 °C/-20 °C
  • Target See all GDNF ELISA Kits

    GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

    Alternative Name

    GDNF

    Gene ID

    25453

    UniProt

    A7UGJ1

    Pathways

    RTK Signaling, Synaptic Membrane, Tube Formation, Autophagy, Smooth Muscle Cell Migration
You are here:
Chat with us!