GDNF ELISA Kit

Catalog No. ABIN411276

The Rat GDNF ELISA Kit (ABIN411276) is a Colorimetric ELISA Kit designed to quantify Rat GDNF. This ELISA Kit has been cited in 9 publications.

Quick Overview for GDNF ELISA Kit (ABIN411276)

Target: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Binding Specificity: AA 78-211

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Product Details GDNF ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Purpose: Sandwich Rat GDNF ELISA Kit to quantitate Rat GDNF in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Analytical Method: Quantitative

Specificity: Expression system for standard: NS0
Immunogen sequence: S78-I211

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 4 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 4.8%, 7.9%, 7.1%
Inter-Assay Precision (CV%): 5.9%, 9.3%, 7.9%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

References for GDNF ELISA Kit (ABIN411276)

Duan, Lu, Wang, Zhang, Mao, Cao, Lin, Zhang, Shuai, Shen: "The long-term fate of mesenchymal stem cells labeled with magnetic resonance imaging-visible polymersomes in cerebral ischemia." in: International journal of nanomedicine, Vol. 12, pp. 6705-6719, (2018) (PubMed).

Sun, Zhu, Yin, Guo, Xu, Xiao, Jiang, Guo, Meng, Lu, Wang, Peng: "Differentiation of adipose-derived stem cells into Schwann cell-like cells through intermittent induction: potential advantage of cellular transient memory function." in: Stem cell research & therapy, Vol. 9, Issue 1, pp. 133, (2018) (PubMed).

Zhuang, Bu, Hua, Darabi, Cao, Xing: "Gelatin-methacrylamide gel loaded with microspheres to deliver GDNF in bilayer collagen conduit promoting sciatic nerve growth." in: International journal of nanomedicine, Vol. 11, pp. 1383-94, (2016) (PubMed).

Ziv-Polat, Shahar, Levy, Skaat, Neuman, Fregnan, Geuna, Grothe, Haastert-Talini, Margel: "The role of neurotrophic factors conjugated to iron oxide nanoparticles in peripheral nerve regeneration: in vitro studies." in: BioMed research international, Vol. 2014, pp. 267808, (2015) (PubMed).

Morano, Wrobel, Fregnan, Ziv-Polat, Shahar, Ratzka, Grothe, Geuna, Haastert-Talini: "Nanotechnology versus stem cell engineering: in vitro comparison of neurite inductive potentials." in: International journal of nanomedicine, Vol. 9, pp. 5289-306, (2015) (PubMed).

Liu, Wang, Shao, Liu: "Genetically modified Schwann cells producing glial cell line-derived neurotrophic factor inhibit neuronal apoptosis in rat spinal cord injury." in: Molecular medicine reports, Vol. 9, Issue 4, pp. 1305-12, (2014) (PubMed).

Banerjee, Nürnberger, Hennerbichler, Riedl, Schuh, Hacobian, Teuschl, Eibl, Redl, Wolbank: "In toto differentiation of human amniotic membrane towards the Schwann cell lineage." in: Cell and tissue banking, Vol. 15, Issue 2, pp. 227-39, (2014) (PubMed).

Chai, Guo, Li, Wang, Wang, Shi, Hu, Liu, Adah: "Scutellarin and caffeic acid ester fraction, active components of Dengzhanxixin injection, upregulate neurotrophins synthesis and release in hypoxia/reoxygenation rat astrocytes." in: Journal of ethnopharmacology, Vol. 150, Issue 1, pp. 100-7, (2013) (PubMed).

Yang, Zhou, Gao, Chen, Tu, Sun, Liu, He, Liu, Yuan: "Neuroprotective effects of bone marrow stem cells overexpressing glial cell line-derived neurotrophic factor on rats with intracerebral hemorrhage and neurons exposed to hypoxia/reoxygenation." in: Neurosurgery, Vol. 68, Issue 3, pp. 691-704, (2011) (PubMed).

Target Details for GDNF

Target: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Alternative Name: GDNF

Background:

Background: Glial cell line-derived neurotrophic factor (GDNF) is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily. GDNF, is a potent neurotrophic factor that promotes the survival of dopaminergic neurones in cultures including embryonic neuronal cultures. GDNF, in addition to its potential role in the differentiation and survival of central nervous system neurons, has profound effects on kidney organogenesis and the development of the peripheral nervous system.3 GDNF may have utility in the treatment of Parkinson's disease, which is marked by progressive degeneration of midbrain dopaminergic neurons. GDNF lies on the short arm of human chromosome 5, at 5p13.1-p13.3 ability to promote dopamine uptake in midbrain cultures. The standard product used in this kit is recombinant rat GDNF, which is a dimmer composed of two chains with 134 amino acids.

Gene Full Name: glial cell derived neurotrophic factor

Gene ID: 25453

UniProt: Q07731

Pathways: RTK Signaling, Synaptic Membrane, Tube Formation, Autophagy, Smooth Muscle Cell Migration