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IL-13 ELISA Kit

Mouse IL-13 ELISA Kit Colorimetric assay for quantification of Mouse IL-13.
Catalog No. ABIN2042524

Quick Overview for IL-13 ELISA Kit (ABIN2042524)

Target

See all IL-13 (IL13) ELISA Kits
IL-13 (IL13) (Interleukin 13 (IL13))

Reactivity

  • 17
  • 12
  • 8
  • 4
  • 4
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
Mouse

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

15.6-1000 pg/mL

Application

ELISA
  • Minimum Detection Limit

    15.6 pg/mL

    Cross-Reactivity (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity

    < 2 pg/mL

    Components

    96-well plate pre-coated with anti-IL-13, Mouse
    Lyophilized recombinant IL-13, Mouse standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • Plate

    Pre-coated

    Protocol

    Sandwich ELISA quantitative detection kit for Mouse IL-13 in cell culture supernates, serum and plasma (heparin, EDTA).Interleukin (IL)-13 is a major inducer of fibrosis in many chronic infectious and autoimmune diseases.1 Recombinant IL-13 protein inhibits inflammatory cytokine production induced by lipopolysaccharide in human peripheral blood monocytes. Moreover, it synergizes with IL-13 in regulating interferon-gamma synthesis in large granular lymphocytes. Interleukin-13 may be critical in regulating inflammatory and immune responses.2 IL-13 and IL-4 are two cytokines produced by T helper type 2 cells, mast cells, and basophils. In addition to their physiological roles, these cytokines are also implicated in pathological conditions such as asthma and allergy.3 The genes encoding human IL-4 and IL-13 are located on segment q23-31 of chromosome 5.4 Recombinant mouse IL-13 is a mixture of Ser26-Phe131 amino acids with the molecular mass of 11.5kD.

    Restrictions

    For Research Use only
  • Storage

    4 °C/-20 °C
  • Target See all IL-13 (IL13) ELISA Kits

    IL-13 (IL13) (Interleukin 13 (IL13))

    Alternative Name

    IL-13

    Gene ID

    16163

    UniProt

    P20109

    Pathways

    JAK-STAT Signaling, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport
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