IL-13 ELISA Kit

Catalog No. ABIN921119

This Colorimetric ELISA kit is designed for the quantitative measurement of Rat IL-13. There are 2 publications available.

Quick Overview for IL-13 ELISA Kit (ABIN921119)

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Binding Specificity: AA 19-131

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6 pg/mL - 1000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Product Details IL-13 ELISA Kit

Minimum Detection Limit: 15.6 pg/mL

Purpose: Sandwich Rat IL-13 ELISA Kit to quantitate Rat Il13 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli
Immunogen sequence: T19-H131

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 1 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 7.4%, 6.1%, 5.5%
Inter-Assay Precision (CV%): 8.6%, 7.9%, 7%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

References for IL-13 ELISA Kit (ABIN921119)

Song, Li, Liang, Dai, Shen, Gong, Wang, Hu, Wang: "Low-level laser facilitates alternatively activated macrophage/microglia polarization and promotes functional recovery after crush spinal cord injury in rats." in: Scientific reports, Vol. 7, Issue 1, pp. 620, (2017) (PubMed).

Huang, Ng, Chen, Lin, Tang, Cheng, Yang, Jiang: "Inhibition of TRPM7 channels reduces degranulation and release of cytokines in rat bone marrow-derived mast cells." in: International journal of molecular sciences, Vol. 15, Issue 7, pp. 11817-31, (2015) (PubMed).

Target Details for IL-13

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Alternative Name: IL13

Background:

Background: Interleukin 13 (IL-13) is a protein that in humans is encoded by the IL13 gene. IL-13 is cytokine secreted by many cell types, but especially T helper type 2 (Th2) cells, that is an important mediator of allergic inflammation and disease. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor (IL-4Ralpha) and at least one of two known IL-13-specific binding chains. Most of the biological effects of IL-13, like those of IL-4, are linked to a single transcription factor, signal transducer and activator of transcription 6 (STAT6). The IL13 gene is clustered with IL3, IL5, IL4, and CSF2 on 5q. Physical maps of the 5q23-q31 region show the following order: cen--IL3--CSF2--IL13--IL4--IL5--tel, with IL13 particularly close to IL4.

Gene Full Name: interleukin 13

Gene ID: 116553

UniProt: P42203

Pathways: JAK-STAT Signaling, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport