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IL-4 ELISA Kit

This Mouse IL-4 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Mouse IL-4.
Catalog No. ABIN2042553

Quick Overview for IL-4 ELISA Kit (ABIN2042553)

Target

See all IL-4 (IL4) ELISA Kits
IL-4 (IL4) (Interleukin 4 (IL4))

Reactivity

  • 20
  • 14
  • 14
  • 6
  • 5
  • 5
  • 5
  • 4
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
Mouse

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

7.8-500 pg/mL

Application

ELISA
  • Minimum Detection Limit

    7.8 pg/mL

    Cross-Reactivity (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity

    < 1 pg/mL

    Components

    96-well plate pre-coated with anti-IL-4, Mouse
    Lyophilized recombinant IL-4, Mouse standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • Plate

    Pre-coated

    Protocol

    Sandwich ELISA quantitative detection kit for Mouse IL-4 in cell culture supernates and serum.Interleukin-4 (IL-4), also knowns as a B-cell stimulatory factor1 (BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells.1 The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-directed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis.1 The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5.2

    Restrictions

    For Research Use only
  • Storage

    4 °C/-20 °C
  • Target See all IL-4 (IL4) ELISA Kits

    IL-4 (IL4) (Interleukin 4 (IL4))

    Alternative Name

    IL-4

    Gene ID

    16189

    UniProt

    P07750

    Pathways

    JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation
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