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Lipocalin 2 ELISA Kit

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse Lipocalin 2.
Catalog No. ABIN2042579

Quick Overview for Lipocalin 2 ELISA Kit (ABIN2042579)

Target

See all Lipocalin 2 (LCN2) ELISA Kits
Lipocalin 2 (LCN2)

Reactivity

  • 21
  • 13
  • 12
  • 5
  • 4
  • 4
  • 4
  • 2
  • 2
  • 2
  • 2
  • 1
Mouse

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

8-5000 pg/mL

Application

ELISA
  • Minimum Detection Limit

    8 pg/mL

    Cross-Reactivity (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity

    < 10 pg/mL

    Components

    96-well plate pre-coated with anti-Lipocalin-2/NGAL, Mouse
    Lyophilized recombinant Lipocalin-2/NGAL, Mouse standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • Plate

    Pre-coated

    Protocol

    Sandwich ELISA quantitative detection kit for Human Mouse Lipocalin-2/NGAL in cell culture supernates, serum, plasma (heparin) and urine.Lipocalin-2 (LCN2), also known as NGAL, is a protein associated with neutrophil gelatinase.1 The LCN2 gene is located at 9q34 and contains 7 exons.2 The 25kD LCN2 protein is believed to bind small lipophilic substances such as bacteria-derived lipopolysaccharide (LPS) and formylpeptides and may function as a modulator of inflammation. NGAL tightly binds bacterial catecholate-type ferric siderophores through a cyclically permuted, hybrid electrostatic/cation-pi interaction and is a potent bacteriostatic agent in iron-limiting conditions.3 The primary LCN2 transcript is 3,696 nucleotides long, and the processed transcript is 809 nucleotides long.4 LCN2 expression in adult bone marrow, uterus, prostate, salivary gland, stomach, appendix, colon, trachea, and lung, and in fetal spleen and lung. The standard product used in this kit is recombinant mouse NGAL, consisting of 179 amino acids with the molecular mass of 22kD.

    Restrictions

    For Research Use only
  • Storage

    4 °C/-20 °C
  • Target See all Lipocalin 2 (LCN2) ELISA Kits

    Lipocalin 2 (LCN2)

    Alternative Name

    Lipocalin-2/NGAL

    Gene ID

    16819

    UniProt

    P11672

    Pathways

    Cellular Response to Molecule of Bacterial Origin, Transition Metal Ion Homeostasis
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