Lipocalin 2 ELISA Kit

Catalog No. ABIN411401

This Colorimetric ELISA kit is designed for the quantitative measurement of Human Lipocalin 2. There are 12 publications available.

Quick Overview for Lipocalin 2 ELISA Kit (ABIN411401)

Target: Lipocalin 2 (LCN2)

Binding Specificity: AA 21-198

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Saliva, Urine

Product Details Lipocalin 2 ELISA Kit

Minimum Detection Limit: 156 pg/mL

Purpose: Sandwich Human Lipocalin-2/NGAL ELISA Kit to quantitate Human LCN2 in cell culture supernatants, serum, plasma (heparin) , saliva and urine.

Analytical Method: Quantitative

Specificity: Expression system for standard: NS0
Immunogen sequence: Q21-G198

Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 7.5%, 5.4%, 5.2%
Inter-Assay Precision (CV%): 7.5%, 5.5%, 6.3%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

References for Lipocalin 2 ELISA Kit (ABIN411401)

Lei, Li, Zeng, Mu, Yang, Zhou, Wang, Zhang: "Value of urinary KIM-1 and NGAL combined with serum Cys C for predicting acute kidney injury secondary to decompensated cirrhosis." in: Scientific reports, Vol. 8, Issue 1, pp. 7962, (2018) (PubMed).

Yigit, Ulu, Gozel, Taskapan, Ilhan, Dogukan: "Neutrophil gelatinase-associated lipocalin reflects the severity of anemia without iron deficiency and secondary hyperparathyroidism in hemodialysis patients." in: Northern clinics of Istanbul, Vol. 4, Issue 1, pp. 36-42, (2017) (PubMed).

Benli, Ayyildiz, Cirrik, Noyan, Ayyildiz, Cirakoglu: "Early term effect of ureterorenoscopy (URS) on the Kidney: research measuring NGAL, KIM-1, FABP and CYS C levels in urine." in: International braz j urol : official journal of the Brazilian Society of Urology, Vol. 43, Issue 5, pp. 887-895, (2017) (PubMed).

Farahbakhsh-Farsi, Djazayery, Eshraghian, Koohdani, Zarei, Javanbakht, Derakhshanian, Djalali: "Effect of Omega-3 Supplementation on Lipocalin 2 and Retinol-Binding Protein 4 in Type 2 Diabetic Patients." in: Iranian journal of public health, Vol. 45, Issue 2, pp. 179-85, (2016) (PubMed).

Libório, Braz, Seguro, Meneses, Neves, Pedrosa, Cavalcanti, Martins, Daher: "Endothelial glycocalyx damage is associated with leptospirosis acute kidney injury." in: The American journal of tropical medicine and hygiene, Vol. 92, Issue 3, pp. 611-6, (2015) (PubMed).

Liu, Dong, Sun, Liu: "A novel fluid resuscitation protocol: provide more protection on acute kidney injury during septic shock in rats." in: International journal of clinical and experimental medicine, Vol. 7, Issue 4, pp. 919-26, (2014) (PubMed).

Merrikhi, Gheissari, Mousazadeh: "Urine and serum neutrophil gelatinase-associated lipocalin cut-off point for the prediction of acute kidney injury." in: Advanced biomedical research, Vol. 3, pp. 66, (2014) (PubMed).

Chen, Lu, Wang, Bian, Cheng, Su, Zhou: "Elevated urinary levels of cystatin C and neutrophil gelatinase-associated lipocalin in Henoch-Schönlein purpura patients with renal involvement." in: PLoS ONE, Vol. 9, Issue 6, pp. e101026, (2014) (PubMed).

Yazdani, Merrikhi, Beni, Baradaran, Soleimani, Musazade: "Association between neutrophil geletinase-associated lipocalin and iron deficiency anemia in children on chronic dialysis." in: Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences, Vol. 19, Issue 7, pp. 624-8, (2014) (PubMed).

Koca, Olguner, Ergür, Altekin, Ta?dö?en, Duru, Girgin, Gündüz, Cilaker M?c?l?, Güzelda?, Akku?: "The effects of dexmedetomidine on secondary acute lung and kidney injuries in the rat model of intra-abdominal sepsis." in: TheScientificWorldJournal, Vol. 2013, pp. 292687, (2013) (PubMed).

Sun, Meng, Jiang, Liu, Lei, Su, Duan, Wu, Xia, Xia: "Protective effect of ginsenoside Rb1 against intestinal ischemia-reperfusion induced acute renal injury in mice." in: PLoS ONE, Vol. 8, Issue 12, pp. e80859, (2013) (PubMed).

Olguner, Koca, Altekin, Ergür, Duru, Girgin, Ta?dö?en, Gündüz, Güzelda?, Akku?, Micili: "Ischemic preconditioning attenuates lipid peroxidation and apoptosis in the cecal ligation and puncture model of sepsis." in: Experimental and therapeutic medicine, Vol. 5, Issue 6, pp. 1581-1588, (2013) (PubMed).

Target Details for Lipocalin 2

Target: Lipocalin 2 (LCN2)

Alternative Name: LCN2

Background:

Background: Lipocalin-2 (LCN2), also known as NGAL, is a protein associated with neutrophil gelatinase.1 The LCN2 gene is located at 9q34 and contains 7 exons.2 The 25-kD LCN2 protein is believed to bind small lipophilic substances such as bacteria-derived lipopolysaccharide (LPS) and formylpeptides and may function as a modulator of inflammation. NGAL tightly binds bacterial catecholate-type ferric siderophores through a cyclically permuted, hybrid electrostatic/cation-pi interaction and is a potent bacteriostatic agent in iron-limiting conditions.3 The primary LCN2 transcript is 3,696 nucleotides long, and the processed transcript is 809 nucleotides long.4 LCN2 expression in adult bone marrow, uterus, prostate, salivary gland, stomach, appendix, colon, trachea, and lung, and in fetal spleen and lung.The standard product used in this kit is recombinant human NGAL, consisting of 178 amino acids with the molecular mass of 22KDa.

Gene Full Name: lipocalin 2

Gene ID: 3934

UniProt: P80188

Pathways: Cellular Response to Molecule of Bacterial Origin, Transition Metal Ion Homeostasis