Lipocalin 2 ELISA Kit
Quick Overview for Lipocalin 2 ELISA Kit (ABIN2042580)
Target
See all Lipocalin 2 (LCN2) ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 6 pg/mL
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Cross-Reactivity (Details)
- There is no detectable cross-reactivity with other relevant proteins.
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Sensitivity
- < 10 pg/mL
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Components
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96-well plate pre-coated with anti-Lipocalin-2/NGAL, Rat
Lyophilized recombinant Lipocalin-2/NGAL, Rat standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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Lipocalin 2 (LCN2) ELISA Kit
LCN2 Reactivity: Human Colorimetric Sandwich ELISA 15.6-1000 pg/mL Plasma, Serum, Tissue Homogenate, Urine
Lipocalin 2 (LCN2) ELISA KitLCN2 Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate, Urine
Lipocalin 2 (LCN2) ELISA KitLCN2 Reactivity: Human Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Lipocalin 2 (LCN2) ELISA KitLCN2 Reactivity: Mouse Colorimetric Competition ELISA 12.5 pg/mL - 800 pg/mL Plasma, Serum, Tissue Homogenate, Urine
Lipocalin 2 (LCN2) ELISA KitLCN2 Reactivity: Rat Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate, Urine
Lipocalin 2 (LCN2) ELISA KitLCN2 Reactivity: Pig Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate, Urine
Lipocalin 2 (LCN2) ELISA KitLCN2 Reactivity: Human AA 21-198 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (heparin), Saliva, Serum, Urine
Lipocalin 2 (LCN2) ELISA KitLCN2 Reactivity: Rat AA 21-198 Colorimetric Sandwich ELISA 78-5000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum, Urine
Lipocalin 2 (LCN2) ELISA KitLCN2 Reactivity: Rat Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Lipocalin 2 (LCN2) ELISA KitLCN2 Reactivity: Mouse Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate, Urine
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Plate
- Pre-coated
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Protocol
- Sandwich ELISA quantitative detection kit for Rat Lipocalin-2/NGAL in cell culture supernates, serum, plasma (heparin) and urine.Lipocalin-2 (LCN2), also known as NGAL, is a protein associated with neutrophil gelatinase.1 The LCN2 gene is located at 9q34 and contains 7 exons.2 The 25kD LCN2 protein is believed to bind small lipophilic substances such as bacteria-derived lipopolysaccharide (LPS) and formylpeptides and may function as a modulator of inflammation. NGAL tightly binds bacterial catecholate-type ferric siderophores through a cyclically permuted, hybrid electrostatic/cation-pi interaction and is a potent bacteriostatic agent in iron-limiting conditions.3 The primary LCN2 transcript is 3,696 nucleotides long, and the processed transcript is 809 nucleotides long.4 LCN2 expression in adult bone marrow, uterus, prostate, salivary gland, stomach, appendix, colon, trachea, and lung, and in fetal spleen and lung. The standard product used in this kit is recombinant rat NGAL, consisting of 177 amino acids with the molecular mass of 21kD.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- Lipocalin 2 (LCN2)
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Alternative Name
- Lipocalin-2/NGAL
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Gene ID
- 170496
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UniProt
- P30152
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Pathways
- Cellular Response to Molecule of Bacterial Origin, Transition Metal Ion Homeostasis
Target See all Lipocalin 2 (LCN2) ELISA Kits
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