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CCL2 ELISA Kit

This Colorimetric ELISA kit is designed for the quantitative measurement of Rat CCL2.
Catalog No. ABIN2042588

Quick Overview for CCL2 ELISA Kit (ABIN2042588)

Target

See all CCL2 ELISA Kits
CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Reactivity

  • 18
  • 18
  • 16
  • 5
  • 5
  • 5
  • 4
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  • 3
  • 3
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  • 2
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  • 2
Rat

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

15.6-1000 pg/mL

Application

ELISA
  • Minimum Detection Limit

    15.6 pg/mL

    Cross-Reactivity (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity

    < 10 pg/mL

    Components

    96-well plate pre-coated with anti-MCP-1/CCL2, Rat
    Lyophilized recombinant MCP-1/CCL2, Rat standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • Plate

    Pre-coated

    Protocol

    Sandwich ELISA quantitative detection kit for Rat MCP-1/CCL2 in cell culture supernates and serum.Monocyte chemoattractant protein-1 (MCP-1), a member of the chemokine (chemotactic cytokine) family, is a potent monocyte agonist that is upregulated by oxidized lipids.1 MCP-1 is also known as CCL2, SCYA2, MCAF. MCAF is a member of family of factors involved in immune and inflammatory responses. The amino acid sequence deduced from the nucleotide sequence reveals the primary structure of the MCAF precursor to be composed of a putative signal peptide sequence of 23 amino acid residues and a mature MCAF sequence of 76 amino acid residues.2 MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder.3

    Restrictions

    For Research Use only
  • Storage

    4 °C/-20 °C
  • Target See all CCL2 ELISA Kits

    CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

    Alternative Name

    MCP-1 (CCL2)

    Gene ID

    24770

    UniProt

    P14844

    Pathways

    Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
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