MMP8 ELISA Kit
Quick Overview for MMP8 ELISA Kit (ABIN2042608)
Target
See all MMP8 ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 6 pg/mL
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Cross-Reactivity (Details)
- There is no detectable cross-reactivity with other relevant proteins.
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Sensitivity
- < 10 pg/mL
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Components
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96-well plate pre-coated with anti-MMP-8, Human
Lyophilized recombinant MMP-8, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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SensoLyte® 490 MMP-8 Assay Kit
FRET Fluorometric
SensoLyte® 520 MMP-8 Assay KitFRET Fluorometric
Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) ELISA KitMMP8 Reactivity: Human Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) ELISA KitMMP8 Reactivity: Mouse Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) ELISA KitMMP8 Reactivity: Rat AA 21-466 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) ELISA KitMMP8 Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) ELISA KitMMP8 Reactivity: Guinea Pig Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) ELISA KitMMP8 Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) ELISA KitMMP8 Reactivity: Rat Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) ELISA KitMMP8 Reactivity: Mouse Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
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Plate
- Pre-coated
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Protocol
- Sandwich ELISA quantitative detection kit for Human MMP-8 in cell culture supernates, serum, plasma (heparin) and saliva.Matrix metalloproteinase 8 (MMP8) also called neutrophil collagenase. Neutrophil collagenase, a member of the family of matrix metalloproteinases, is distinct from the collagenase of skin fibroblasts and synovial cells in substrate specificity and immunologic crossreactivity. MMP8, an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells.1 The human neutrophil collagenase (HNC) cDNA clone has been sequenced and shown to encode a 467-residue protein.2 Neutrophil collagenase has been found to possess 57% identity with the deduced protein sequence for fibroblast collagenase with 72% chemical similarity. Certain regions of the molecule, including the putative zinc-binding region, are highly conserved. When compared with the published sequence for fibroblast collagenase, neutrophil collagenase contains four additional sites for glycosylation.3 The standard product used in this kit is natural, isolating from human MMP-8. The detected MMP-8 includes zymogen and active enzyme.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))
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Alternative Name
- MMP-8
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Gene ID
- 4317
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UniProt
- P22894
Target See all MMP8 ELISA Kits
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