MME ELISA Kit

Catalog No. ABIN2042615

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse MME.

Quick Overview for MME ELISA Kit (ABIN2042615)

Target: MME (Membrane Metallo-Endopeptidase (MME))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 93.7-6000 pg/mL

Application: ELISA

Product Details MME ELISA Kit

Minimum Detection Limit: 93.7 pg/mL

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components: 96-well plate pre-coated with anti-Neprilysin/CD10, Mouse
Lyophilized recombinant Neprilysin/CD10, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Application Details

Plate: Pre-coated

Protocol: Sandwich ELISA quantitative detection kit for Mouse Neprilysin/CD10 in cell culture supernates, serum and plasma (heparin, EDTA).Neprilysin, also known as membrane metallo-endopeptidase, neutral endopeptidase (NEP), CD10, and common acute lymphoblastic leukemia antigen (CALLA), is a zinc-dependent metalloprotease enzyme that degrades a number of small secreted peptides, most notably theamyloid beta peptide whose abnormal misfolding and aggregation in neural tissue has been implicated as a cause of Alzheimer's disease, this gene is localized to human chromosome 3 by study of somatic cell hybrids and regionalized the location to 3q21-q27 by in situ hybridization. By cDNA transfection analysis, Neprilysin is confirmed as a functional neutral endopeptidase of the type that has previously been called enkephalinase. Neprilysin has also been called atriopeptidase. Atriopeptidase specifically degrades atrial natriuretic factor. A specific enzyme inhibitor was developed and reported that it had effects similar to those of low-dose ANF infusion. These effects include diuresis, natriuresis, vasodilatation, and suppression of the renin-angiotensin-aldosterone system.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for MME

Target: MME (Membrane Metallo-Endopeptidase (MME))

Alternative Name: Neprilysin/CD10

Target Type: Chemical

Gene ID: 17380

UniProt: Q61391

Pathways: RTK Signaling, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Smooth Muscle Cell Migration