ACAT1 ELISA Kit

Catalog No. ABIN2042749

Product Details ACAT1 ELISA Kit

Target: ACAT1 (Acetyl-CoA Acetyltransferase 1 (ACAT1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Minimum Detection Limit: 0.15 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.052 ng/mL

Application Details

Plate: Pre-coated

Protocol: Acetyl Coenzyme A Acetyltransferase 1 (ACAT1) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Acetyl Coenzyme A Acetyltransferase 1 (ACAT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Acetyl Coenzyme A Acetyltransferase 1 (ACAT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Acetyl Coenzyme A Acetyltransferase 1 (ACAT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Acetyl Coenzyme A Acetyltransferase 1 (ACAT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for ACAT1

Target: ACAT1 (Acetyl-CoA Acetyltransferase 1 (ACAT1))

Alternative Name: Acetyl Coenzyme A Acetyltransferase 1 (ACAT1) (ACAT1 Products)

Synonyms: ACAT ELISA Kit, MAT ELISA Kit, T2 ELISA Kit, THIL ELISA Kit, RATACAL ELISA Kit, 6330585C21Rik ELISA Kit, Acat ELISA Kit, fd16h07 ELISA Kit, fd20g06 ELISA Kit, wu:fd16h07 ELISA Kit, wu:fd20g06 ELISA Kit, zgc:86832 ELISA Kit, acat1-a ELISA Kit, acetyl-CoA acetyltransferase 1 ELISA Kit, acetyl-Coenzyme A acetyltransferase 1 ELISA Kit, acetyl-CoA acetyltransferase 1 L homeolog ELISA Kit, ACAT1 ELISA Kit, Acat1 ELISA Kit, acat1 ELISA Kit, acat1.L ELISA Kit