APEX1 ELISA Kit

Catalog No. ABIN2042915

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse APEX1.

Quick Overview for APEX1 ELISA Kit (ABIN2042915)

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details APEX1 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.051 ng/mL

Application Details

Plate: Pre-coated

Protocol: APEX Nuclease 1 (APEX1) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to APEX Nuclease 1 (APEX1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to APEX Nuclease 1 (APEX1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain APEX Nuclease 1 (APEX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of APEX Nuclease 1 (APEX1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for APEX1

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Alternative Name: APEX Nuclease 1 (APEX1)

Pathways: DNA Damage Repair, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus