BIM ELISA Kit

Catalog No. ABIN2042999

This Colorimetric ELISA kit is designed for the quantitative measurement of Human BIM.

Quick Overview for BIM ELISA Kit (ABIN2042999)

Target: BIM (BCL2L11) (BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details BIM ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.114 ng/mL

Application Details

Plate: Pre-coated

Protocol: Bcl2 Like Protein 11 (BCL2L11) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bcl2 Like Protein 11 (BCL2L11). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bcl2 Like Protein 11 (BCL2L11). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bcl2 Like Protein 11 (BCL2L11), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Bcl2 Like Protein 11 (BCL2L11) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for BIM

Target: BIM (BCL2L11) (BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11))

Alternative Name: Bcl2 Like Protein 11 (BCL2L11)

Pathways: PI3K-Akt Signaling, Neurotrophin Signaling Pathway, Tube Formation, Positive Regulation of Endopeptidase Activity