FBLIM1 ELISA Kit

Catalog No. ABIN2043485

Product Details FBLIM1 ELISA Kit

Target: FBLIM1 (Filamin Binding LIM Protein 1 (FBLIM1))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Minimum Detection Limit: 0.15 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.058 ng/mL

Application Details

Plate: Pre-coated

Protocol: Filamin Binding LIM Protein 1 (FBLIM1) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Filamin Binding LIM Protein 1 (FBLIM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Filamin Binding LIM Protein 1 (FBLIM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Filamin Binding LIM Protein 1 (FBLIM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Filamin Binding LIM Protein 1 (FBLIM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for FBLIM1

Target: FBLIM1 (Filamin Binding LIM Protein 1 (FBLIM1))

Abstract: FBLIM1 Products

Synonyms: FBLIM1 ELISA Kit, CAL ELISA Kit, FBLP-1 ELISA Kit, FBLP1 ELISA Kit, 2410043F08Rik ELISA Kit, Cal ELISA Kit, Fblp1 ELISA Kit, Gt10 ELISA Kit, migfilin ELISA Kit, migfilin(s) ELISA Kit, filamin binding LIM protein 1 ELISA Kit, filamin binding LIM protein 1 S homeolog ELISA Kit, FBLIM1 ELISA Kit, fblim1.S ELISA Kit, Fblim1 ELISA Kit