FUCA2 ELISA Kit

Catalog No. ABIN2043515

This Colorimetric ELISA kit is designed for the quantitative measurement of Human FUCA2.

Quick Overview for FUCA2 ELISA Kit (ABIN2043515)

Target: FUCA2 (Fucosidase, alpha-L- 2, Plasma (FUCA2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.62-40 ng/mL

Application: ELISA

Product Details FUCA2 ELISA Kit

Minimum Detection Limit: 0.62 ng/mL

Sensitivity: < 0.216 ng/mL

Application Details

Plate: Pre-coated

Protocol: Fucosidase Alpha L2, Plasma (FUCa2) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fucosidase Alpha L2, Plasma (FUCa2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fucosidase Alpha L2, Plasma (FUCa2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fucosidase Alpha L2, Plasma (FUCa2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Fucosidase Alpha L2, Plasma (FUCa2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for FUCA2

Target: FUCA2 (Fucosidase, alpha-L- 2, Plasma (FUCA2))

Alternative Name: Fucosidase alpha L2, Plasma (FUCa2)