FUCA2 ELISA Kit

Catalog No. ABIN5654438

Product Details FUCA2 ELISA Kit

Target: FUCA2 (Fucosidase, alpha-L- 2, Plasma (FUCA2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.62 pg/mL - 1000 pg/mL

Minimum Detection Limit: 15.62 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Fucosidase Alpha L2, Plasma (FUCa2). No significant cross-reactivity or interference between Fucosidase Alpha L2, Plasma (FUCa2) and analogues was observed.

Sensitivity: 6.8 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fucosidase Alpha L2, Plasma (FUCa2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fucosidase Alpha L2, Plasma (FUCa2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fucosidase Alpha L2, Plasma (FUCa2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fucosidase Alpha L2, Plasma (FUCa2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fucosidase Alpha L2, Plasma (FUCa2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fucosidase Alpha L2, Plasma (FUCa2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for FUCA2

Target: FUCA2 (Fucosidase, alpha-L- 2, Plasma (FUCA2))

Alternative Name: Fucosidase Alpha L2, Plasma (FUCA2 Products)

Synonyms: MGC68702 ELISA Kit, zgc:92013 ELISA Kit, wu:fc20c06 ELISA Kit, F10M10.30 ELISA Kit, F10M10_30 ELISA Kit, SMb20490 ELISA Kit, dJ20N2.5 ELISA Kit, 0610025O11Rik ELISA Kit, 5530401P20Rik ELISA Kit, alpha-L-fucosidase 2 L homeolog ELISA Kit, fucosidase, alpha-L- 2, plasma ELISA Kit, alpha-L-fucosidase 2 ELISA Kit, 1,2-alpha-L-fucosidase ELISA Kit, methylthioribulose-1-phosphate dehydratase ELISA Kit, fuculose-1-phosphate aldolase ELISA Kit, L-fuculose phosphate aldolase ELISA Kit, fuca2.L ELISA Kit, FUCA2 ELISA Kit, fuca2 ELISA Kit, FUC95A ELISA Kit, LOC100282758 ELISA Kit, LOC100284864 ELISA Kit, LOC100284892 ELISA Kit, LOC100285541 ELISA Kit, LOC100285863 ELISA Kit, LOC100286039 ELISA Kit, fucA2 ELISA Kit, Fuca2 ELISA Kit

Background:

Gene Name: Fucosidase Alpha L2, Plasma

Gene Aliases: Alpha-L-fucoside fucohydrolase 2

Gene ID: 2519

UniProt: Q9BTY2