G6PC ELISA Kit

Catalog No. ABIN2043553

Mouse G6PC ELISA Kit, Colorimetric assay for quantification of Mouse G6PC.

Quick Overview for G6PC ELISA Kit (ABIN2043553)

Target: G6PC (Glucose 6-Phosphatase, Catalytic (G6PC))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56-100 ng/mL

Application: ELISA

Product Details G6PC ELISA Kit

Minimum Detection Limit: 1.56 ng/mL

Sensitivity: < 0.52 ng/mL

Application Details

Plate: Pre-coated

Protocol: Glucose-6-Phosphatase, Catalytic (G6PC) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucose-6-Phosphatase, Catalytic (G6PC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glucose-6-Phosphatase, Catalytic (G6PC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucose-6-Phosphatase, Catalytic (G6PC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Glucose-6-Phosphatase, Catalytic (G6PC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for G6PC

Target: G6PC (Glucose 6-Phosphatase, Catalytic (G6PC))

Alternative Name: Glucose-6-Phosphatase, Catalytic (G6PC)

Pathways: Carbohydrate Homeostasis, Cellular Glucan Metabolic Process