GP1BB ELISA Kit

Catalog No. ABIN2043583

Mouse GP1BB ELISA Kit Colorimetric assay for quantification of Mouse GP1BB.

Quick Overview for GP1BB ELISA Kit (ABIN2043583)

Target: GP1BB (Glycoprotein Ib (Platelet), beta Polypeptide (GP1BB))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.25-2000 pg/mL

Application: ELISA

Product Details GP1BB ELISA Kit

Minimum Detection Limit: 31.25 pg/mL

Brand: BioAssay™

Sensitivity: < 14.7 pg/mL

Application Details

Plate: Pre-coated

Protocol: Glycoprotein Ib Beta Polypeptide, Platelet (GP1Bb) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycoprotein Ib Beta Polypeptide, Platelet (GP1Bb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glycoprotein Ib Beta Polypeptide, Platelet (GP1Bb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycoprotein Ib Beta Polypeptide, Platelet (GP1Bb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Glycoprotein Ib Beta Polypeptide, Platelet (GP1Bb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for GP1BB

Target: GP1BB (Glycoprotein Ib (Platelet), beta Polypeptide (GP1BB))

Alternative Name: Glycoprotein Ib beta Polypeptide, Platelet (GP1Bb)