IL-2 ELISA Kit

Catalog No. ABIN2043675

The Human IL-2 ELISA Kit (ABIN2043675) is a Colorimetric ELISA Kit designed to quantify Human IL-2.

Quick Overview for IL-2 ELISA Kit (ABIN2043675)

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.78-50 pg/mL

Application: ELISA

Product Details IL-2 ELISA Kit

Minimum Detection Limit: 0.78 pg/mL

Sensitivity: < 0.28 pg/mL

Application Details

Plate: Pre-coated

Protocol: High Sensitivity Interleukin 2 (IL2) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 2 (IL2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 2 (IL2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 2 (IL2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Interleukin 2 (IL2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for IL-2

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation