IL-13 ELISA Kit

Catalog No. ABIN2043803

This Colorimetric ELISA kit is designed for the quantitative measurement of Cow IL-13.

Quick Overview for IL-13 ELISA Kit (ABIN2043803)

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Reactivity: Cow

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 7.81-500 pg/mL

Application: ELISA

Product Details IL-13 ELISA Kit

Minimum Detection Limit: 7.81 pg/mL

Sensitivity: < 2.7 pg/mL

Application Details

Plate: Pre-coated

Protocol: Interleukin 13 (IL13) BioAssay-ELISA Kit (Bovine) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 13 (IL13). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 13 (IL13). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 13 (IL13), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Interleukin 13 (IL13) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for IL-13

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Pathways: JAK-STAT Signaling, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport