JAK3 ELISA Kit

Catalog No. ABIN2043827

This Colorimetric ELISA kit is designed for the quantitative measurement of Human JAK3.

Quick Overview for JAK3 ELISA Kit (ABIN2043827)

Target: JAK3 (Janus Kinase 3 (JAK3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details JAK3 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.066 ng/mL

Application Details

Plate: Pre-coated

Protocol: Janus Kinase 3 (JAK3) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Janus Kinase 3 (JAK3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Janus Kinase 3 (JAK3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Janus Kinase 3 (JAK3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Janus Kinase 3 (JAK3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for JAK3

Target: JAK3 (Janus Kinase 3 (JAK3))

Pathways: JAK-STAT Signaling, RTK Signaling, Response to Growth Hormone Stimulus, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus, Activated T Cell Proliferation, Unfolded Protein Response