Kallikrein 13 ELISA Kit

Catalog No. ABIN2043841

Mouse Kallikrein 13 ELISA Kit Colorimetric assay for quantification of Mouse Kallikrein 13.

Quick Overview for Kallikrein 13 ELISA Kit (ABIN2043841)

Target: Kallikrein 13 (KLK13) (Kallikrein-Related Peptidase 13 (KLK13))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.62-1000 pg/mL

Application: ELISA

Product Details Kallikrein 13 ELISA Kit

Minimum Detection Limit: 15.62 pg/mL

Brand: BioAssay™

Sensitivity: < 6.9 pg/mL

Application Details

Plate: Pre-coated

Protocol: Kallikrein 13 (KLK13) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kallikrein 13 (KLK13). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kallikrein 13 (KLK13). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kallikrein 13 (KLK13), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Kallikrein 13 (KLK13) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Kallikrein 13

Target: Kallikrein 13 (KLK13) (Kallikrein-Related Peptidase 13 (KLK13))

Alternative Name: Kallikrein 13 (KLK13)

Pathways: Complement System