HLA-A ELISA Kit
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- Target See all HLA-A ELISA Kits
- HLA-A (Major Histocompatibility Complex, Class I, A (HLA-A))
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.31-20 ng/mL
- Minimum Detection Limit
- 0.31 ng/mL
- Application
- ELISA
- Brand
- BioAssay™
- Sensitivity
- < 0.126 ng/mL
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- Discover our best selling HLA-A ELISA Kit
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- Discover our top product HLA-A ELISA Kit
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- Plate
- Pre-coated
- Protocol
- Major Histocompatibility Complex Class I A (MHCA) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Major Histocompatibility Complex Class I A (MHCA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Major Histocompatibility Complex Class I A (MHCA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Major Histocompatibility Complex Class I A (MHCA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Major Histocompatibility Complex Class I A (MHCA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Restrictions
- For Research Use only
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- Storage
- 4 °C/-20 °C
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- Target See all HLA-A ELISA Kits
- HLA-A (Major Histocompatibility Complex, Class I, A (HLA-A))
- Alternative Name
- Major Histocompatibility Complex Class I A (MHCA) (HLA-A Products)
- Synonyms
- HLAA ELISA Kit, major histocompatibility complex, class I, A ELISA Kit, HLA-A ELISA Kit
- Pathways
- TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response
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