HLA-B ELISA Kit

Catalog No. ABIN2043915

This Human HLA-B ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human HLA-B.

Quick Overview for HLA-B ELISA Kit (ABIN2043915)

Target: HLA-B (Major Histocompatibility Complex, Class I, B (HLA-B))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56-100 ng/mL

Application: ELISA

Product Details HLA-B ELISA Kit

Minimum Detection Limit: 1.56 ng/mL

Brand: BioAssay™

Sensitivity: < 0.63 ng/mL

Application Details

Plate: Pre-coated

Protocol: Major Histocompatibility Complex Class I B (MHCB) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Major Histocompatibility Complex Class I B (MHCB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Major Histocompatibility Complex Class I B (MHCB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Major Histocompatibility Complex Class I B (MHCB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Major Histocompatibility Complex Class I B (MHCB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for HLA-B

Target: HLA-B (Major Histocompatibility Complex, Class I, B (HLA-B))

Alternative Name: Major Histocompatibility Complex Class I B (MHCB)

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response