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HLA-B ELISA Kit (Major Histocompatibility Complex, Class I, B) ELISA Kit

HLA-B Reactivity: Human Colorimetric Sandwich ELISA 1.56-100 ng/mL
Catalog No. ABIN2043915
$982.14
Plus shipping costs $45.00
96 tests
local_shipping Shipping to: United States
Delivery in 16 to 21 Business Days
  • Target
    Reactivity
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    1.56-100 ng/mL
    Minimum Detection Limit
    1.56 ng/mL
    Application
    ELISA
    Brand
    BioAssay™
    Sensitivity
    < 0.63 ng/mL
  • Target
    Alternative Name
    Major Histocompatibility Complex Class I B (MHCB) (HLA-B ELISA Kit Abstract)
    Pathways
    Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response
  • Plate
    Pre-coated
    Protocol
    Major Histocompatibility Complex Class I B (MHCB) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Major Histocompatibility Complex Class I B (MHCB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Major Histocompatibility Complex Class I B (MHCB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Major Histocompatibility Complex Class I B (MHCB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Major Histocompatibility Complex Class I B (MHCB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Restrictions
    For Research Use only
  • Storage
    4 °C/-20 °C
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