NOTCH2 ELISA Kit

Catalog No. ABIN2044111

Human NOTCH2 ELISA Kit, Colorimetric assay for quantification of Human NOTCH2.

Quick Overview for NOTCH2 ELISA Kit (ABIN2044111)

Target: NOTCH2 (Notch 2 (NOTCH2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.78-50 ng/mL

Application: ELISA

Product Details NOTCH2 ELISA Kit

Minimum Detection Limit: 0.78 ng/mL

Sensitivity: < 0.33 ng/mL

Application Details

Plate: Pre-coated

Protocol: Notch Homolog 2 (NOTCH2) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Notch Homolog 2 (NOTCH2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Notch Homolog 2 (NOTCH2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Notch Homolog 2 (NOTCH2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Notch Homolog 2 (NOTCH2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for NOTCH2

Target: NOTCH2 (Notch 2 (NOTCH2))

Alternative Name: Notch Homolog 2 (NOTCH2)

Pathways: Notch Signaling, Stem Cell Maintenance