OGG1 ELISA Kit

Catalog No. ABIN2044157

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse OGG1.

Quick Overview for OGG1 ELISA Kit (ABIN2044157)

Target: OGG1 (8-Oxoguanine DNA Glycosylase (OGG1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.25-2000 pg/mL

Application: ELISA

Product Details OGG1 ELISA Kit

Minimum Detection Limit: 31.25 pg/mL

Sensitivity: < 12.1 pg/mL

Application Details

Plate: Pre-coated

Protocol: Oxoguanine Glycosylase 1 (OGG1) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Oxoguanine Glycosylase 1 (OGG1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Oxoguanine Glycosylase 1 (OGG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Oxoguanine Glycosylase 1 (OGG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Oxoguanine Glycosylase 1 (OGG1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for OGG1

Target: OGG1 (8-Oxoguanine DNA Glycosylase (OGG1))

Alternative Name: Oxoguanine Glycosylase 1 (OGG1)

Pathways: DNA Damage Repair