RAD23B ELISA Kit

Catalog No. ABIN2044377

The Human RAD23B ELISA Kit (ABIN2044377) is a Colorimetric ELISA Kit designed to quantify Human RAD23B.

Quick Overview for RAD23B ELISA Kit (ABIN2044377)

Target: RAD23B (RAD23 Homolog B (RAD23B))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details RAD23B ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.052 ng/mL

Application Details

Plate: Pre-coated

Protocol: RAD23 Homolog B (RAD23B) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RAD23 Homolog B (RAD23B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to RAD23 Homolog B (RAD23B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RAD23 Homolog B (RAD23B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of RAD23 Homolog B (RAD23B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for RAD23B

Target: RAD23B (RAD23 Homolog B (RAD23B))

Pathways: DNA Damage Repair