RIPK1 ELISA Kit

Catalog No. ABIN2044401

The Rat RIPK1 ELISA Kit (ABIN2044401) is a Colorimetric ELISA Kit designed to quantify Rat RIPK1.

Quick Overview for RIPK1 ELISA Kit (ABIN2044401)

Target: RIPK1 (Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details RIPK1 ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.112 ng/mL

Application Details

Plate: Pre-coated

Protocol: Receptor Interacting Serine Threonine Kinase 1 (RIPK1) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Receptor Interacting Serine Threonine Kinase 1 (RIPK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Receptor Interacting Serine Threonine Kinase 1 (RIPK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Receptor Interacting Serine Threonine Kinase 1 (RIPK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Receptor Interacting Serine Threonine Kinase 1 (RIPK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for RIPK1

Target: RIPK1 (Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1))

Alternative Name: Receptor Interacting Serine Threonine Kinase 1 (RIPK1)

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Activation of Innate immune Response, Inositol Metabolic Process, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Negative Regulation of intrinsic apoptotic Signaling, SARS-CoV-2 Protein Interactome, Ubiquitin Proteasome Pathway